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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
International journal of biological macromolecules
2025
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40306517/ |
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| author | Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna |
| author_facet | Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna |
| collection | PubMed - marine biology |
| contents | Structural insights and bioactivity of a cell wall sulfated polysaccharide from the marine diatom Cyclotella cryptica. Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna Diatoms Polysaccharides Cell Wall Sulfates Antiviral Agents Herpesvirus 1, Human Humans Sulfated Polysaccharides (SPs) are abundant in marine organisms, where they play an essential role in the mechanisms of adaptation to saline environments. SPs from macro- and microalgae possess unique structural features, which often correlate to taxonomy, and are under active investigation due to their various potential fields of application. We have investigated the structure of the sulfated polysaccharide isolated from the cell wall of the marine diatom Cyclotella cryptica (CcSP), finding that it consisted of a homopolysaccharide with a backbone of (1 → 4)-⍺-d-Manp carrying, in the most abundant form, sulfation at O-6. CcSP exhibited promising antiviral activity against Herpes Simplex Virus-1 (HSV-1), which was likely associated with a mechanism involving steric hindrance and/or electrostatic repulsion, preventing viral attachment to host cells. In addition, we have also proved the binding of CcSP to the innate human receptor Langerin, a well-known C-type lectin that recognizes sulfated polysaccharides and is involved in virus entry in cells. We attempted to partially reconstruct the biosynthetic pathway of CcSP: analysis of C. cryptica genome revealed the presence of several putative carbohydrate 6-O sulfotransferases (CH-STs) with homology to the human enzymes involved in glycosaminoglycans sulfation. Our results suggest an ancient evolutionary origin for the regioselective specialization of CH-STs, and could pave the way for future research on diatom cell wall biogenesis, as well as for biotechnological applications relying on the manipulation of the sulfation levels in CcSP for enhanced activity. |
| format | Artículo científico |
| id | pubmed_40306517 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Structural insights and bioactivity of a cell wall sulfated polysaccharide from the marine diatom Cyclotella cryptica. Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna Diatoms Polysaccharides Cell Wall Sulfates Antiviral Agents Herpesvirus 1, Human Humans Structural insights and bioactivity of a cell wall sulfated polysaccharide from the marine diatom Cyclotella cryptica. Leone, Serena Giugliano, Rosa Borgonuovo, Camilla Chiodo, Fabrizio Molinaro, Antonio Zannella, Carla De Filippis, Anna Galdiero, Massimiliano van Kooyk, Yvette De Chiara, Stefania Magnabosco, Chiara Di Lorenzo, Flaviana Romano, Giovanna Diatoms Polysaccharides Cell Wall Sulfates Antiviral Agents Herpesvirus 1, Human Humans Sulfated Polysaccharides (SPs) are abundant in marine organisms, where they play an essential role in the mechanisms of adaptation to saline environments. SPs from macro- and microalgae possess unique structural features, which often correlate to taxonomy, and are under active investigation due to their various potential fields of application. We have investigated the structure of the sulfated polysaccharide isolated from the cell wall of the marine diatom Cyclotella cryptica (CcSP), finding that it consisted of a homopolysaccharide with a backbone of (1 → 4)-⍺-d-Manp carrying, in the most abundant form, sulfation at O-6. CcSP exhibited promising antiviral activity against Herpes Simplex Virus-1 (HSV-1), which was likely associated with a mechanism involving steric hindrance and/or electrostatic repulsion, preventing viral attachment to host cells. In addition, we have also proved the binding of CcSP to the innate human receptor Langerin, a well-known C-type lectin that recognizes sulfated polysaccharides and is involved in virus entry in cells. We attempted to partially reconstruct the biosynthetic pathway of CcSP: analysis of C. cryptica genome revealed the presence of several putative carbohydrate 6-O sulfotransferases (CH-STs) with homology to the human enzymes involved in glycosaminoglycans sulfation. Our results suggest an ancient evolutionary origin for the regioselective specialization of CH-STs, and could pave the way for future research on diatom cell wall biogenesis, as well as for biotechnological applications relying on the manipulation of the sulfation levels in CcSP for enhanced activity. |
| title | Structural insights and bioactivity of a cell wall sulfated polysaccharide from the marine diatom Cyclotella cryptica. |
| topic | Diatoms Polysaccharides Cell Wall Sulfates Antiviral Agents Herpesvirus 1, Human Humans |
| url | https://pubmed.ncbi.nlm.nih.gov/40306517/ |