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| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Insects
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40332822/ |
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Table of Contents:
- Additive Insecticidal Effects of Chitosan/dsRNA Nanoparticles Targeting and Emamectin Benzoate-Lufenuron Formulations Against (J.E. Smith) (Lepidoptera: Noctuidae). Guo, Shigang Li, Zhongwei Zhao, Xuhui Zhang, Donghai Ayra-Pardo, Camilo Kan, Yunchao Li, Dandan The fall armyworm, , a lepidopteran pest from the family Noctuidae, has become a major invasive pest since 2016. Using RNAi methods to control is currently under investigation. This study is the first to target the gene of using RNAi. Injection of dsRNA-V-ATPaseD into the hemolymph of 4th-instar larvae significantly suppressed gene expression at 24 and 48 h post-injection. Treated larvae showed delayed development and reduced pupation after 7 days. Subsequently, silencing was achieved through topical or oral administration of chitosan/dsRNA-V-ATPaseD nanoparticles. Larvae fed these nanoparticles exhibited significant reductions in mRNA at 72 h, persisting until 96 h before normalizing. Additionally, the treated larvae displayed disrupted molting and impaired pupation. Furthermore, larvae fed chitosan/dsRNA-V-ATPaseD were more susceptible to emamectin benzoate-lufenuron at LC30 concentrations, resulting in 68% mortality-27% higher than the pesticide alone-72 h post-exposure. Combining chitosan/dsRNA-V-ATPaseD nanoparticles with emamectin benzoate-lufenuron significantly enhanced pest control efficacy, providing new insights into pesticide reduction and sustainable pest control methods for this invasive species.