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| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40335686/ |
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| _version_ | 1868266206570479617 |
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| author | Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi |
| author_facet | Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi |
| collection | PubMed - marine biology |
| contents | Bioremediation of complex organic pollutants by engineered Vibrio natriegens. Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi Vibrio Biodegradation, Environmental Wastewater Multigene Family Genetic Engineering Synthetic Biology Organic Chemicals Petroleum Industrial Waste Water Pollutants, Chemical Petroleum Pollution Industrial wastewater, petroleum pollution and plastic contamination are significant threats to global marine biosecurity because of their toxic, mutagenic and persistent nature. The use of microorganisms in bioremediation has been constrained by the complexity of organic pollutants and limited tolerance to saline stress. In this study, we used synthetic biology to engineer Vibrio natriegens into a strain capable of bioremediating complex organic pollutants in saline wastewater and soils. The competence master regulator gene tfoX was inserted into chromosome 1 of the V. natriegens strain Vmax and overexpressed to enhance DNA uptake and integration. Degradation gene clusters were chemically synthesized and assembled in yeast. We developed a genome engineering method (iterative natural transformation based on Vmax with amplified tfoX effect) to transfer five gene clusters (43 kb total) into Vmax. The engineered strain has the ability to bioremediate five organic pollutants (biphenyl, phenol, naphthalene, dibenzofuran and toluene) covering a broad substrate range, from monocyclic to multicyclic compounds, in industrial wastewater samples from a chlor-alkali plant and a petroleum refinery. |
| format | Artículo científico |
| id | pubmed_40335686 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature |
| record_format | pubmed |
| spellingShingle | Bioremediation of complex organic pollutants by engineered Vibrio natriegens. Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi Vibrio Biodegradation, Environmental Wastewater Multigene Family Genetic Engineering Synthetic Biology Organic Chemicals Petroleum Industrial Waste Water Pollutants, Chemical Petroleum Pollution Bioremediation of complex organic pollutants by engineered Vibrio natriegens. Su, Cong Cui, Haotian Wang, Weiwei Liu, Yong Cheng, Zhenyu Wang, Chen Yang, Mengqiao Qu, Liwen Li, Ye Cai, Yuejin He, Siyang Zheng, Jiaxin Zhao, Pingping Xu, Ping Dai, Junbiao Tang, Hongzhi Vibrio Biodegradation, Environmental Wastewater Multigene Family Genetic Engineering Synthetic Biology Organic Chemicals Petroleum Industrial Waste Water Pollutants, Chemical Petroleum Pollution Industrial wastewater, petroleum pollution and plastic contamination are significant threats to global marine biosecurity because of their toxic, mutagenic and persistent nature. The use of microorganisms in bioremediation has been constrained by the complexity of organic pollutants and limited tolerance to saline stress. In this study, we used synthetic biology to engineer Vibrio natriegens into a strain capable of bioremediating complex organic pollutants in saline wastewater and soils. The competence master regulator gene tfoX was inserted into chromosome 1 of the V. natriegens strain Vmax and overexpressed to enhance DNA uptake and integration. Degradation gene clusters were chemically synthesized and assembled in yeast. We developed a genome engineering method (iterative natural transformation based on Vmax with amplified tfoX effect) to transfer five gene clusters (43 kb total) into Vmax. The engineered strain has the ability to bioremediate five organic pollutants (biphenyl, phenol, naphthalene, dibenzofuran and toluene) covering a broad substrate range, from monocyclic to multicyclic compounds, in industrial wastewater samples from a chlor-alkali plant and a petroleum refinery. |
| title | Bioremediation of complex organic pollutants by engineered Vibrio natriegens. |
| topic | Vibrio Biodegradation, Environmental Wastewater Multigene Family Genetic Engineering Synthetic Biology Organic Chemicals Petroleum Industrial Waste Water Pollutants, Chemical Petroleum Pollution |
| url | https://pubmed.ncbi.nlm.nih.gov/40335686/ |