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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of applied microbiology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40336148/ |
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Table of Contents:
- Metabologenomics analysis reveals antibiotic crypticity of Kutzneria viridogrisea DSM 43850. Dong, Bing-Cheng Liu, Fen Hu, Gang-Ao Yu, Wen-Chao Li, Zi-Yang Guan, Yu-Tian Zhang, Wen-Wen Wang, Hong Wei, Bin Metabolomics Anti-Bacterial Agents Multigene Family Genome, Bacterial Whole Genome Sequencing Secondary Metabolism Computational Biology This study aimed to explore the secondary metabolic potential of Kutzneria viridogrisea DSM 43850 by conducting whole-genome sequencing and utilizing bioinformatics tools to analyze its biosynthetic gene clusters (BGCs). Additionally, the secondary metabolites produced by this strain were investigated under various chemical elicitors using untargeted metabolomics techniques. The complete genome of K. viridogrisea DSM 43850 was obtained by re-sequencing, followed by in-depth bioinformatics analysis to assess its secondary metabolic potential. The genome was found to encode a circular 10.2 Mb chromosome, with 4.3% of its functional genes involved in secondary metabolism. The strain harbors 52 BGCs, of which only 4 are associated with known products. Among these, eight gene clusters were identified as ribosomally synthesized and post-translationally modified peptides, and the precursor peptide structures of four were predicted, all featuring novel scaffolds. Untargeted metabolomics analysis using liquid chromatography-mass spectrometry revealed that the strain could produce a series of novel secondary metabolites when induced with kanamycin and an ebselen derivative. This study highlights the significant secondary metabolic potential of K. viridogrisea DSM 43850, uncovering several novel BGCs and metabolic products.