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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40347992/ |
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| _version_ | 1868266206553702400 |
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| author | Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini |
| author_facet | Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini |
| collection | PubMed - marine biology |
| contents | Molecular characterization and functional analysis of IL-18 in large yellow croaker (Larimichthys crocea). Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini Animals Perciformes Fish Diseases Fish Proteins Phylogeny Interleukin-18 Amino Acid Sequence Sequence Alignment Gene Expression Regulation Vibrio alginolyticus Immunity, Innate Vibrio Infections Gene Expression Profiling Pseudomonas Base Sequence Interleukin-18 (IL-18), a pro-inflammatory cytokine of the IL-1 family, is crucial for protecting the host against pathogen infection in mammals. In this study, a IL-18 homolog gene was cloned from large yellow croaker (Larimichthys crocea) (LcIL-18), which has an open reading frame (ORF) of 609 bp that encodes a polypeptide of 202 amino acids. The LcIL-18 C-terminus contains a typical IL-1 family signature and a caspase cleavage site. Phylogenetic analysis showed that LcIL-18 was most closely related to IL-18 of Miichthys miiuy. It was found that LcIL-18 was constitutively expressed in all 12 tissues tested of large yellow croakers, with the highest expression in gills. The expression of LcIL-18 in head kidney, spleen, skin, gills, and liver showed a differential pattern following infection with Pseudomonas plecoglossicida and Vibrio alginolyticus. P. plecoglossicida strongly induced LcIL-18 expression in these tissues. Conversely, in the early stage of infection, V. alginolyticus significantly inhibited LcIL-18 expression in head kidney, spleen, skin, and gills, but not in the liver. In vitro, LPS, Poly(I:C), P. plecoglossicida, and V. alginolyticus significantly upregulated the expression of the LcIL-18 in large yellow croaker head kidney (LYCK) cells. Furthermore, recombinant LcIL-18 (rLcIL-18) significantly increased cell viability and upregulated the expression of pro-inflammatory cytokines (LcIL-1β, LcIL-6, and LcTNF-α1) in LYCK cells. Our findings therefore indicated that LcIL-18 was involved in pro-inflammatory response induced by pathogenic bacteria. |
| format | Artículo científico |
| id | pubmed_40347992 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Molecular characterization and functional analysis of IL-18 in large yellow croaker (Larimichthys crocea). Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini Animals Perciformes Fish Diseases Fish Proteins Phylogeny Interleukin-18 Amino Acid Sequence Sequence Alignment Gene Expression Regulation Vibrio alginolyticus Immunity, Innate Vibrio Infections Gene Expression Profiling Pseudomonas Base Sequence Molecular characterization and functional analysis of IL-18 in large yellow croaker (Larimichthys crocea). Wang, Yongyang Ye, Jiajia Luo, Yufen Weng, Zufeng Lu, Qi Xia, Changchang Ma, Fanfan Kang, Fuyu Chen, Xinhua Zhang, Weini Animals Perciformes Fish Diseases Fish Proteins Phylogeny Interleukin-18 Amino Acid Sequence Sequence Alignment Gene Expression Regulation Vibrio alginolyticus Immunity, Innate Vibrio Infections Gene Expression Profiling Pseudomonas Base Sequence Interleukin-18 (IL-18), a pro-inflammatory cytokine of the IL-1 family, is crucial for protecting the host against pathogen infection in mammals. In this study, a IL-18 homolog gene was cloned from large yellow croaker (Larimichthys crocea) (LcIL-18), which has an open reading frame (ORF) of 609 bp that encodes a polypeptide of 202 amino acids. The LcIL-18 C-terminus contains a typical IL-1 family signature and a caspase cleavage site. Phylogenetic analysis showed that LcIL-18 was most closely related to IL-18 of Miichthys miiuy. It was found that LcIL-18 was constitutively expressed in all 12 tissues tested of large yellow croakers, with the highest expression in gills. The expression of LcIL-18 in head kidney, spleen, skin, gills, and liver showed a differential pattern following infection with Pseudomonas plecoglossicida and Vibrio alginolyticus. P. plecoglossicida strongly induced LcIL-18 expression in these tissues. Conversely, in the early stage of infection, V. alginolyticus significantly inhibited LcIL-18 expression in head kidney, spleen, skin, and gills, but not in the liver. In vitro, LPS, Poly(I:C), P. plecoglossicida, and V. alginolyticus significantly upregulated the expression of the LcIL-18 in large yellow croaker head kidney (LYCK) cells. Furthermore, recombinant LcIL-18 (rLcIL-18) significantly increased cell viability and upregulated the expression of pro-inflammatory cytokines (LcIL-1β, LcIL-6, and LcTNF-α1) in LYCK cells. Our findings therefore indicated that LcIL-18 was involved in pro-inflammatory response induced by pathogenic bacteria. |
| title | Molecular characterization and functional analysis of IL-18 in large yellow croaker (Larimichthys crocea). |
| topic | Animals Perciformes Fish Diseases Fish Proteins Phylogeny Interleukin-18 Amino Acid Sequence Sequence Alignment Gene Expression Regulation Vibrio alginolyticus Immunity, Innate Vibrio Infections Gene Expression Profiling Pseudomonas Base Sequence |
| url | https://pubmed.ncbi.nlm.nih.gov/40347992/ |