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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40350021/ |
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| _version_ | 1868266204031877121 |
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| author | He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming |
| author_facet | He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming |
| collection | PubMed - marine biology |
| contents | Non-allergenic mixed T cell epitope peptides for preventing Scy p 9 allergic reactions. He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming Animals Mice Humans Epitopes, T-Lymphocyte Immunoglobulin E Female Mice, Inbred BALB C Peptides Allergens Brachyura Male Shellfish Hypersensitivity Arthropod Proteins Filamin c is an allergen with strong IgE-binding activity of Scylla paramamosain (named Scy p 9). In this study, the T cell epitopes of Scy p 9 were identified using the CD4T lymphocyte proliferation assay in the mononuclear cells of the spleen allergic mice. Nine of the thirteen T cell epitope peptides that have been found have the ability to trigger the production of the Th1 cytokine (IFN-γ) and TGF-β. Meanwhile, the findings of the inhibitory Dot blot and basophil activation test of crab allergy patients showed that the mixed nine T cell epitope peptides lacked IgE-binding activity and was unable to stimulate the expression of CD63 and CD203c on the surface of basophils. Non-allergenic mixed T cell epitope peptides (NAMTEP) demonstrate the potential for preventing Scy p 9 allergies. According to the evaluation of the preventive effect of Scy p 9 allergy mouse model, mice challenged with oral administration of Scy p 9 after early injection of NAMTEP did not show any allergic symptoms. NAMTEP inhibited IgE generation and Th2 immune response, preventing Scy p 9 allergy reactions in mice. These results would provide a theoretical foundation for the specific prevention of allergens in crustacean aquatic products. |
| format | Artículo científico |
| id | pubmed_40350021 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association |
| record_format | pubmed |
| spellingShingle | Non-allergenic mixed T cell epitope peptides for preventing Scy p 9 allergic reactions. He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming Animals Mice Humans Epitopes, T-Lymphocyte Immunoglobulin E Female Mice, Inbred BALB C Peptides Allergens Brachyura Male Shellfish Hypersensitivity Arthropod Proteins Non-allergenic mixed T cell epitope peptides for preventing Scy p 9 allergic reactions. He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming Animals Mice Humans Epitopes, T-Lymphocyte Immunoglobulin E Female Mice, Inbred BALB C Peptides Allergens Brachyura Male Shellfish Hypersensitivity Arthropod Proteins Filamin c is an allergen with strong IgE-binding activity of Scylla paramamosain (named Scy p 9). In this study, the T cell epitopes of Scy p 9 were identified using the CD4T lymphocyte proliferation assay in the mononuclear cells of the spleen allergic mice. Nine of the thirteen T cell epitope peptides that have been found have the ability to trigger the production of the Th1 cytokine (IFN-γ) and TGF-β. Meanwhile, the findings of the inhibitory Dot blot and basophil activation test of crab allergy patients showed that the mixed nine T cell epitope peptides lacked IgE-binding activity and was unable to stimulate the expression of CD63 and CD203c on the surface of basophils. Non-allergenic mixed T cell epitope peptides (NAMTEP) demonstrate the potential for preventing Scy p 9 allergies. According to the evaluation of the preventive effect of Scy p 9 allergy mouse model, mice challenged with oral administration of Scy p 9 after early injection of NAMTEP did not show any allergic symptoms. NAMTEP inhibited IgE generation and Th2 immune response, preventing Scy p 9 allergy reactions in mice. These results would provide a theoretical foundation for the specific prevention of allergens in crustacean aquatic products. |
| title | Non-allergenic mixed T cell epitope peptides for preventing Scy p 9 allergic reactions. |
| topic | Animals Mice Humans Epitopes, T-Lymphocyte Immunoglobulin E Female Mice, Inbred BALB C Peptides Allergens Brachyura Male Shellfish Hypersensitivity Arthropod Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/40350021/ |