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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40350021/ |
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Table of Contents:
- Non-allergenic mixed T cell epitope peptides for preventing Scy p 9 allergic reactions. He, Xinrong Han, Xinyu Yang, Yang Li, Fajie Li, Dongxiao Luo, Lianzhong Liu, Hong Chen, Guixia Chen, Xiaomei Liu, Guangming Animals Mice Humans Epitopes, T-Lymphocyte Immunoglobulin E Female Mice, Inbred BALB C Peptides Allergens Brachyura Male Shellfish Hypersensitivity Arthropod Proteins Filamin c is an allergen with strong IgE-binding activity of Scylla paramamosain (named Scy p 9). In this study, the T cell epitopes of Scy p 9 were identified using the CD4T lymphocyte proliferation assay in the mononuclear cells of the spleen allergic mice. Nine of the thirteen T cell epitope peptides that have been found have the ability to trigger the production of the Th1 cytokine (IFN-γ) and TGF-β. Meanwhile, the findings of the inhibitory Dot blot and basophil activation test of crab allergy patients showed that the mixed nine T cell epitope peptides lacked IgE-binding activity and was unable to stimulate the expression of CD63 and CD203c on the surface of basophils. Non-allergenic mixed T cell epitope peptides (NAMTEP) demonstrate the potential for preventing Scy p 9 allergies. According to the evaluation of the preventive effect of Scy p 9 allergy mouse model, mice challenged with oral administration of Scy p 9 after early injection of NAMTEP did not show any allergic symptoms. NAMTEP inhibited IgE generation and Th2 immune response, preventing Scy p 9 allergy reactions in mice. These results would provide a theoretical foundation for the specific prevention of allergens in crustacean aquatic products.