Preparation of Chitooligosaccharides with Specific Sequence Arrangement and Their Effect on Inducing Salt Resistance in Wheat Seedlings.
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Polymers
2025
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| _version_ | 1868266204010905600 |
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| author | Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng |
| author_facet | Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng |
| collection | PubMed - marine biology |
| contents | Preparation of Chitooligosaccharides with Specific Sequence Arrangement and Their Effect on Inducing Salt Resistance in Wheat Seedlings. Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng Chitooligosaccharides (COS) exhibits good activity of inducing plant resistance, but the structure-activity relationship is still unclear. In this study, chitin oligosaccharides (CHOS) with a degree of polymerization (DP) of 2~6 were used as raw materials. Three deacetylases (NodB, COD, and CE4A) were employed to prepare three different sequence-arranged COSs, namely N-COS, C-COS, and A-COS, and their structures were characterized by infrared spectroscopy, high-performance liquid chromatography, and mass spectrometry. Further studies were conducted on inducing the plant salt resistance of the three different sequence-arranged COSs on wheat seedlings. The results showed a sequence-dependent effect of COS inducing plant salt resistance. Among them, A-COS exhibited the best activity. When sprayed at a concentration of 10 mg/L on wheat seedlings under salt stress for 3 days, the leaf length of the wheat seedlings sprayed with A-COS was recovered, and the wet mass and dry mass were recovered by 20.40% and 6.64%, respectively. Following the enhancement of proline accumulation, the malondialdehyde content decreased by 34.75%, and the Na/K ratio also exhibited a significant reduction, thereby alleviating salt stress-induced damage. This study was the first to demonstrate the effect of COS with specific sequences on inducing plant salt resistance, providing a theoretical basis for the development of a new generation of efficient COS plant biostimulator. |
| format | Artículo científico |
| id | pubmed_40362979 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Polymers |
| record_format | pubmed |
| spellingShingle | Preparation of Chitooligosaccharides with Specific Sequence Arrangement and Their Effect on Inducing Salt Resistance in Wheat Seedlings. Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng Preparation of Chitooligosaccharides with Specific Sequence Arrangement and Their Effect on Inducing Salt Resistance in Wheat Seedlings. Li, Jingwen Li, Anbang Li, Yupeng Zhu, Siqi Song, Lin Liu, Song Xing, Ronge Li, Kecheng Chitooligosaccharides (COS) exhibits good activity of inducing plant resistance, but the structure-activity relationship is still unclear. In this study, chitin oligosaccharides (CHOS) with a degree of polymerization (DP) of 2~6 were used as raw materials. Three deacetylases (NodB, COD, and CE4A) were employed to prepare three different sequence-arranged COSs, namely N-COS, C-COS, and A-COS, and their structures were characterized by infrared spectroscopy, high-performance liquid chromatography, and mass spectrometry. Further studies were conducted on inducing the plant salt resistance of the three different sequence-arranged COSs on wheat seedlings. The results showed a sequence-dependent effect of COS inducing plant salt resistance. Among them, A-COS exhibited the best activity. When sprayed at a concentration of 10 mg/L on wheat seedlings under salt stress for 3 days, the leaf length of the wheat seedlings sprayed with A-COS was recovered, and the wet mass and dry mass were recovered by 20.40% and 6.64%, respectively. Following the enhancement of proline accumulation, the malondialdehyde content decreased by 34.75%, and the Na/K ratio also exhibited a significant reduction, thereby alleviating salt stress-induced damage. This study was the first to demonstrate the effect of COS with specific sequences on inducing plant salt resistance, providing a theoretical basis for the development of a new generation of efficient COS plant biostimulator. |
| title | Preparation of Chitooligosaccharides with Specific Sequence Arrangement and Their Effect on Inducing Salt Resistance in Wheat Seedlings. |
| url | https://pubmed.ncbi.nlm.nih.gov/40362979/ |