Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
EvoDevo
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40380248/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266203983642624 |
|---|---|
| author | Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S |
| author_facet | Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S |
| collection | PubMed - marine biology |
| contents | Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata. Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S Cnidarians have become valuable models for understanding many aspects of developmental biology including the evolution of body plan diversity, novel cell type specification, and regeneration. Most of our understanding of gene function during early development in cnidarians comes from a small number of experimental systems including Hydra and the sea anemone, Nematostella vectensis. Few molecular tools have been developed for use in hard corals, limiting our understanding of this diverse and ecologically important clade. Here, we report the development of a suite of tools for manipulating and analyzing gene expression during early development in the northern star coral, Astrangia poculata. We present methods for gene knockdown using short hairpin RNAs, gene overexpression using exogenous mRNAs, and endogenous gene tagging using CRISPR-mediated gene knock-in. Combined with the fact that spawning can be induced in the laboratory, during the reproductive window, these tools make A. poculata a tractable experimental system for investigative studies of coral development. Further application of these tools will enable functional analyses of embryonic patterning and morphogenesis across Anthozoa and open new frontiers in coral biology research. |
| format | Artículo científico |
| id | pubmed_40380248 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | EvoDevo |
| record_format | pubmed |
| spellingShingle | Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata. Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata. Warner, Jacob F Besemer, Ryan Schickle, Alicia Borbee, Erin Changsut, Isabella V Sharp, Koty Babonis, Leslie S Cnidarians have become valuable models for understanding many aspects of developmental biology including the evolution of body plan diversity, novel cell type specification, and regeneration. Most of our understanding of gene function during early development in cnidarians comes from a small number of experimental systems including Hydra and the sea anemone, Nematostella vectensis. Few molecular tools have been developed for use in hard corals, limiting our understanding of this diverse and ecologically important clade. Here, we report the development of a suite of tools for manipulating and analyzing gene expression during early development in the northern star coral, Astrangia poculata. We present methods for gene knockdown using short hairpin RNAs, gene overexpression using exogenous mRNAs, and endogenous gene tagging using CRISPR-mediated gene knock-in. Combined with the fact that spawning can be induced in the laboratory, during the reproductive window, these tools make A. poculata a tractable experimental system for investigative studies of coral development. Further application of these tools will enable functional analyses of embryonic patterning and morphogenesis across Anthozoa and open new frontiers in coral biology research. |
| title | Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata. |
| url | https://pubmed.ncbi.nlm.nih.gov/40380248/ |