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| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
GigaByte (Hong Kong, China)
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40395690/ |
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| _version_ | 1868266201719767040 |
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| author | Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong |
| author_facet | Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong |
| collection | PubMed - marine biology |
| contents | Chromosome-level genome assemblies of five species. Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong , a genus of tetraploid fishes endemic to Southwest China's karst regions, are classified as second-class nationally protected species due to their fragile habitat. Limited high-quality genomic resources have hampered studies on their phylogenetic relationships and the origin of their polyploidy. Here, we present a high-quality genome assembly of the most abundant species, the golden-line barbel (), by integrating PacBio long-read and Hi-C sequencing. The resulting scaffold-level genome-assembly is 1.6 Gb long, with a scaffold N50 of up to 30.7 Mb. We annotated 42,806 protein-coding genes. Also, 93.1% of the assembled genome sequences (about 1.5 Gb) and 93.8% of the total predicted genes were successfully anchored onto 48 chromosomes. Furthermore, we obtained chromosome-level genome assemblies for four other species (, , , and ) based on homologous comparisons. These genomic resources will enable in-depth investigations on cave adaptation, improvement of economic values, and conservation of diverse fishes. |
| format | Artículo científico |
| id | pubmed_40395690 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | GigaByte (Hong Kong, China) |
| record_format | pubmed |
| spellingShingle | Chromosome-level genome assemblies of five species. Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong Chromosome-level genome assemblies of five species. Bian, Chao Li, Ruihan Ouyang, Yuqian Yang, Junxing Mu, Xidong Shi, Qiong , a genus of tetraploid fishes endemic to Southwest China's karst regions, are classified as second-class nationally protected species due to their fragile habitat. Limited high-quality genomic resources have hampered studies on their phylogenetic relationships and the origin of their polyploidy. Here, we present a high-quality genome assembly of the most abundant species, the golden-line barbel (), by integrating PacBio long-read and Hi-C sequencing. The resulting scaffold-level genome-assembly is 1.6 Gb long, with a scaffold N50 of up to 30.7 Mb. We annotated 42,806 protein-coding genes. Also, 93.1% of the assembled genome sequences (about 1.5 Gb) and 93.8% of the total predicted genes were successfully anchored onto 48 chromosomes. Furthermore, we obtained chromosome-level genome assemblies for four other species (, , , and ) based on homologous comparisons. These genomic resources will enable in-depth investigations on cave adaptation, improvement of economic values, and conservation of diverse fishes. |
| title | Chromosome-level genome assemblies of five species. |
| url | https://pubmed.ncbi.nlm.nih.gov/40395690/ |