Provirus deletion from affects motility, stress resistance, and CRISPR RNA expression.
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| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
microLife
2025
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| author | Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita |
| author_facet | Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita |
| collection | PubMed - marine biology |
| contents | Provirus deletion from affects motility, stress resistance, and CRISPR RNA expression. Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3, and Halfvol4. In this study, we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild-type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to HO stress compared to the wild type. RNA-seq also revealed downregulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2, and Halfvol3 were found in the culture supernatant of the wild-type strain. This confirms excision of the proviruses from the chromosome, which seems to happen more efficiently at low temperature (30°C). Electron microscopy revealed potential viral particles in the supernatant, and mass spectrometry analysis confirmed the presence of structural viral proteins of Halfvol1 and Halfvol3 in the isolated virus sample. These observations suggest that these proviruses are active and cause a chronic infection in . |
| format | Artículo científico |
| id | pubmed_40395997 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | microLife |
| record_format | pubmed |
| spellingShingle | Provirus deletion from affects motility, stress resistance, and CRISPR RNA expression. Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita Provirus deletion from affects motility, stress resistance, and CRISPR RNA expression. Di Cianni, Nadia Bolsinger, Simon Brendel, Jutta Raabe, Monika König, Sabine Mitchell, Laura Bischler, Thorsten Gräfenhan, Tom Read, Clarissa Erdmann, Susanne Allers, Thorsten Walther, Paul Urlaub, Henning Dyall-Smith, Mike Pfeiffer, Friedhelm Marchfelder, Anita harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3, and Halfvol4. In this study, we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild-type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to HO stress compared to the wild type. RNA-seq also revealed downregulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2, and Halfvol3 were found in the culture supernatant of the wild-type strain. This confirms excision of the proviruses from the chromosome, which seems to happen more efficiently at low temperature (30°C). Electron microscopy revealed potential viral particles in the supernatant, and mass spectrometry analysis confirmed the presence of structural viral proteins of Halfvol1 and Halfvol3 in the isolated virus sample. These observations suggest that these proviruses are active and cause a chronic infection in . |
| title | Provirus deletion from affects motility, stress resistance, and CRISPR RNA expression. |
| url | https://pubmed.ncbi.nlm.nih.gov/40395997/ |