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| Autori principali: | , , , , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Cell reports
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40397573/ |
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| _version_ | 1868266201715572736 |
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| author | Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai |
| author_facet | Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai |
| collection | PubMed - marine biology |
| contents | Regulation of the non-selective Na importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis. Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai Arabidopsis Phosphorylation Arabidopsis Proteins Sodium Xenopus laevis Animals Oocytes Gene Expression Regulation, Plant Sodium-Hydrogen Exchangers Salt Tolerance Plant Roots Potassium Anion Transport Proteins Protein Serine-Threonine Kinases Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO-promoted Na, K, and Li importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na import and preventing excessive Na accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation. |
| format | Artículo científico |
| id | pubmed_40397573 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Cell reports |
| record_format | pubmed |
| spellingShingle | Regulation of the non-selective Na importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis. Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai Arabidopsis Phosphorylation Arabidopsis Proteins Sodium Xenopus laevis Animals Oocytes Gene Expression Regulation, Plant Sodium-Hydrogen Exchangers Salt Tolerance Plant Roots Potassium Anion Transport Proteins Protein Serine-Threonine Kinases Regulation of the non-selective Na importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis. Liu, Xiaoxiao Zhang, Lei Zhao, Zeqi Zheng, Yue Ren, Yue Zhao, Xiaomin Zhang, Shizhong Yang, Guodong Huang, Jinguang Yan, Kang Li, Chunlong Zheng, Chengchao Wu, Changai Arabidopsis Phosphorylation Arabidopsis Proteins Sodium Xenopus laevis Animals Oocytes Gene Expression Regulation, Plant Sodium-Hydrogen Exchangers Salt Tolerance Plant Roots Potassium Anion Transport Proteins Protein Serine-Threonine Kinases Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO-promoted Na, K, and Li importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na import and preventing excessive Na accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation. |
| title | Regulation of the non-selective Na importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis. |
| topic | Arabidopsis Phosphorylation Arabidopsis Proteins Sodium Xenopus laevis Animals Oocytes Gene Expression Regulation, Plant Sodium-Hydrogen Exchangers Salt Tolerance Plant Roots Potassium Anion Transport Proteins Protein Serine-Threonine Kinases |
| url | https://pubmed.ncbi.nlm.nih.gov/40397573/ |