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| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine life science & technology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40417251/ |
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Table of Contents:
- Molecular networking reveals indole diterpenoids from the marine-derived fungus sp. N4-3. Chen, Min Hao, Bao-Cong Zhu, Xia-Hao Zhang, Li-Kui Zheng, Yao-Yao Zhou, Xiao-Jian Schäberle, Till F Shen, Li Wang, Chang-Yun Liu, Yang MS/MS-based molecular networking is an effective strategy to rapidly dereplicate known compounds and to guide the discovery process for new and novel natural products. In the present study, the chemical diversity of indole diterpenoids from the marine-derived fungus sp. N4-3 was investigated using molecular networking techniques. Guided by this information, targeted isolation resulted in two new indole diterpenoids shearinines R and S (, ) and an oxidative artifact shearinine T (), together with the verification of two known analogs (, ). Furthermore, five indole diterpenoids (-), including three putatively new ones, shearinines U-W (, , ), were predicted from the molecular ion cluster by the combination of GNPS molecular networking and manual analysis of MS/MS fragmentation clusters. Shearinines T () and W () are characterized by an oxidative cleavage of the C-2-C-18 double bond. Feature fragment ions of these shearinines revealed two type of dominant ions related to the indole moiety and the breaking of C-9 side chain or Ring I. Compound showed antibacterial activities against a panel of pathogenic bacteria with IC values ranging from 6.34 to 47.96 μg/mL and inhibited the growth of the human hepatic (HepG2) and gastric (SGC-7901) cancer cells lines with IC values of 6.27 and 19.16 μg/mL, respectively. The online version contains supplementary material available at 10.1007/s42995-024-00274-6.