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| Format: | Artículo científico |
| Language: | en |
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Proceedings of the National Academy of Sciences of the United States of America
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40424131/ |
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| author | Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi |
| author_facet | Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi |
| collection | PubMed - marine biology |
| contents | Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast. Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi Schizosaccharomyces Endoplasmic Reticulum Schizosaccharomyces pombe Proteins Fatty Acids Coenzyme A Ligases Lipidomics Excess amounts of saturated fatty acids (FAs) are toxic to organisms, a condition termed lipotoxicity, which is often accompanied by pleiotropic cellular and tissue dysfunction. Here, we show that pentadecanoic acid (C15:0) exerts toxicity on the fission yeast by generating an aberrantly planar endoplasmic reticulum (ER) structure, which we named a "giant ER sheet." Untargeted lipidomics revealed that C15:0 is incorporated into complex lipids depending on an acyl-CoA ligase Lcf1 and an acyl-CoA transferase Slc1, thereby increasing the saturation level of the acyl chains. The toxicity and giant ER sheet formation were abolished by deleting or gene, indicating that the incorporation of C15:0 into glycerophospholipids causes giant ER sheet formation. The giant ER sheets disrupted the correct migration of Mid1, a protein determining the cell division site, and physically blocked septum formation, hindering correct cell separation. Our results suggest that the ER is the primary site targeted by saturated FAs, leading to lipotoxicity. |
| format | Artículo científico |
| id | pubmed_40424131 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Proceedings of the National Academy of Sciences of the United States of America |
| record_format | pubmed |
| spellingShingle | Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast. Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi Schizosaccharomyces Endoplasmic Reticulum Schizosaccharomyces pombe Proteins Fatty Acids Coenzyme A Ligases Lipidomics Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast. Hoshikawa, Yojiro Shirota, Natsuho Tsugawa, Hiroshi Kimura, Satoshi Matsuyama, Akihisa Yashiroda, Yoko Kakeya, Hideaki Arita, Makoto Iizumi, Reiko Yoshida, Minoru Nishimura, Shinichi Schizosaccharomyces Endoplasmic Reticulum Schizosaccharomyces pombe Proteins Fatty Acids Coenzyme A Ligases Lipidomics Excess amounts of saturated fatty acids (FAs) are toxic to organisms, a condition termed lipotoxicity, which is often accompanied by pleiotropic cellular and tissue dysfunction. Here, we show that pentadecanoic acid (C15:0) exerts toxicity on the fission yeast by generating an aberrantly planar endoplasmic reticulum (ER) structure, which we named a "giant ER sheet." Untargeted lipidomics revealed that C15:0 is incorporated into complex lipids depending on an acyl-CoA ligase Lcf1 and an acyl-CoA transferase Slc1, thereby increasing the saturation level of the acyl chains. The toxicity and giant ER sheet formation were abolished by deleting or gene, indicating that the incorporation of C15:0 into glycerophospholipids causes giant ER sheet formation. The giant ER sheets disrupted the correct migration of Mid1, a protein determining the cell division site, and physically blocked septum formation, hindering correct cell separation. Our results suggest that the ER is the primary site targeted by saturated FAs, leading to lipotoxicity. |
| title | Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast. |
| topic | Schizosaccharomyces Endoplasmic Reticulum Schizosaccharomyces pombe Proteins Fatty Acids Coenzyme A Ligases Lipidomics |
| url | https://pubmed.ncbi.nlm.nih.gov/40424131/ |