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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Genes
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40428409/ |
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Table of Contents:
- Comparative Transcriptome Analysis Elucidates the Desiccation Stress Adaptation in . Cao, Wei Zhang, Mingyi Wu, Nan Zheng, Yanxin Li, Xiaodong Han, Haiying Yu, Tao Wu, Zhongxun Qu, Pei Li, Bo Sargassum Transcriptome Gene Expression Profiling Stress, Physiological Adaptation, Physiological Desiccation Desiccation profoundly influences the distribution and abundance of intertidal seaweeds, necessitating robust molecular adaptations. is a brown seaweed inhabiting intertidal rocky substrates. During low tides, this species undergoes periodic aerial exposure. Such environmental conditions necessitate robust physiological mechanisms to mitigate desiccation stress. Yet, the molecular basis of this adaptation remains poorly understood. To investigate desiccation-responsive genes and elucidate the underlying mechanisms of adaptation, we exposed to 6 h of controlled desiccation stress in sterilized ceramic trays, simulating natural tidal conditions, and performed comparative transcriptome analysis using RNA-seq on the Illumina NovaSeq 6000 platform. High-quality sequencing identified 66,192 unigenes, with 1990 differentially expressed genes (1399 upregulated and 591 downregulated). These differentially expressed genes (DEGs) were categorized into regulatory genes-including mitogen-activated protein kinase (), calmodulin, elongation factor, and serine/threonine-protein kinase-and functional genes, such as heat shock protein family members (, , and ), tubulin ( and ), and endoplasmic reticulum homeostasis-related genes (protein disulfide-isomerase A6, calreticulin, and calnexin). Gene Ontology (GO) enrichment highlighted upregulated DEGs in metabolic processes like glutathione metabolism, critical for oxidative stress mitigation, while downregulated genes were linked to transport functions, such as ammonium transport, suggesting reduced nutrient uptake during dehydration. KEGG pathway analysis revealed significant enrichment in "protein processing in endoplasmic reticulum" and "MAPK signaling pathway-plant", implicating endoplasmic reticulum stress response and conserved signaling cascades in desiccation adaptation. Validation via qRT-PCR confirmed consistent expression trends for key genes, reinforcing the reliability of transcriptomic data. These findings suggest that undergoes extensive biological adjustments to mitigate desiccation stress, highlighting candidate pathways for future investigations into recovery and tolerance mechanisms.