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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of molecular sciences
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40429756/ |
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Table of Contents:
- Identification and Functional Analysis of a Key Gene in the CHH Gene Family for Glucose Metabolism in the Pacific White Shrimp . Zhang, Chengyi Zhang, Xiaojun Si, Shuqing Sun, Mingzhe Li, Aixin Yuan, Jianbo Li, Fuhua Animals Penaeidae Arthropod Proteins Glucose Invertebrate Hormones Phylogeny Nerve Tissue Proteins Multigene Family Alternative Splicing Signal Transduction Gene Expression Regulation The crustacean hyperglycemic hormone (CHH) is a unique multifunctional neuroendocrine hormone superfamily in crustaceans, crucial for maintaining physiological homeostasis and stress adaptation. To explore the role of CHHs in shrimp metabolism and growth, we identified , a CHH family member who regulates glucose metabolism in the Pacific white shrimp (), through CHH family gene classification, phylogenetic analysis, gene structure analysis, and transcription factor binding site (TFBS) prediction. Subsequently, we cloned two alternative splicing variants of this gene, and , both expressed in the nervous system but with different expression levels, and exhibiting a broader tissue distribution. Using interference (RNAi)-mediated gene silencing and recombinant protein injection, we investigated the functional similarities and differences between the two variants. Our results show that both variants affect glucose metabolism by modulating the expression of key enzyme genes involved in gluconeogenesis/glycolysis, such as , , , . Specifically, they likely regulate hemolymph glucose levels via the Wnt and PI3K-AKT signaling pathways, with exerting a more sustained effect on glucose metabolism compared to . Furthermore, LvCHH Ia may also act as a molting inhibitory hormone by suppressing the expression of ecdysone synthesis-related genes, where LvCHH Ia-2 plays a more significant role. These findings deepen our understanding of CHH regulatory mechanisms in crustaceans and provide potential applications for shrimp physiological research and aquaculture.