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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature microbiology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40442502/ |
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| author | Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded |
| author_facet | Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded |
| collection | PubMed - marine biology |
| contents | Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea. Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded Archaea Light Rhodopsins, Microbial Rhodopsin Carotenoids Energy Transfer Metagenome Aquatic Organisms Phylogeny Light-Harvesting Protein Complexes Seawater Archaeal Proteins Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea. |
| format | Artículo científico |
| id | pubmed_40442502 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature microbiology |
| record_format | pubmed |
| spellingShingle | Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea. Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded Archaea Light Rhodopsins, Microbial Rhodopsin Carotenoids Energy Transfer Metagenome Aquatic Organisms Phylogeny Light-Harvesting Protein Complexes Seawater Archaeal Proteins Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea. Tzlil, Gali Marín, María Del Carmen Matsuzaki, Yuma Nag, Probal Itakura, Shota Mizuno, Yosuke Murakoshi, Shunya Tanaka, Tatsuki Larom, Shirley Konno, Masae Abe-Yoshizumi, Rei Molina-Márquez, Ana Bárcenas-Pérez, Daniela Cheel, José Koblížek, Michal León, Rosa Katayama, Kota Kandori, Hideki Schapiro, Igor Shihoya, Wataru Nureki, Osamu Inoue, Keiichi Rozenberg, Andrey Chazan, Ariel Béjà, Oded Archaea Light Rhodopsins, Microbial Rhodopsin Carotenoids Energy Transfer Metagenome Aquatic Organisms Phylogeny Light-Harvesting Protein Complexes Seawater Archaeal Proteins Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea. |
| title | Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea. |
| topic | Archaea Light Rhodopsins, Microbial Rhodopsin Carotenoids Energy Transfer Metagenome Aquatic Organisms Phylogeny Light-Harvesting Protein Complexes Seawater Archaeal Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/40442502/ |