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| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature communications
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40461452/ |
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| author | Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja |
| author_facet | Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja |
| collection | PubMed - marine biology |
| contents | Polyketide synthase-derived sphingolipids mediate microbiota protection against a bacterial pathogen in C. elegans. Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja Caenorhabditis elegans Animals Polyketide Synthases Sphingolipids Bacillus thuringiensis Pseudomonas fluorescens Gastrointestinal Microbiome Caenorhabditis elegans Proteins Serine C-Palmitoyltransferase Host-Pathogen Interactions Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components of host-microbiota interactions, their exact role in microbiota-mediated protection is largely unexplored. We addressed this knowledge gap with the nematode Caenorhabditis elegans and its microbiota isolate Pseudomonas fluorescens MYb115 that is known to protect against Bacillus thuringiensis (Bt) infection. We find that MYb115-mediated protection depends on sphingolipids (SLs) that are derived from an iterative type I polyketide synthase (PKS) cluster PfSgaAB, thereby revealing a non-canonical pathway for the production of bacterial SLs as secondary metabolites. SL production is common in eukaryotes but was thought to be limited to a few bacterial phyla that encode the serine palmitoyltransferase (SPT) enzyme, which catalyses the initial step in SL synthesis. We demonstrate that PfSgaB encodes a pyridoxal 5'-phosphate-dependent alpha-oxoamine synthase with SPT activity, and find homologous putative PKS clusters present across host-associated bacteria that are so far unknown SL producers. Moreover, we provide evidence that MYb115-derived SLs affect C. elegans defence against Bt infection by altering SL metabolism in the nematode host. This work establishes SLs as structural outputs of bacterial PKS and highlights the role of microbiota-derived SLs in host protection against pathogens. |
| format | Artículo científico |
| id | pubmed_40461452 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Polyketide synthase-derived sphingolipids mediate microbiota protection against a bacterial pathogen in C. elegans. Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja Caenorhabditis elegans Animals Polyketide Synthases Sphingolipids Bacillus thuringiensis Pseudomonas fluorescens Gastrointestinal Microbiome Caenorhabditis elegans Proteins Serine C-Palmitoyltransferase Host-Pathogen Interactions Polyketide synthase-derived sphingolipids mediate microbiota protection against a bacterial pathogen in C. elegans. Peters, Lena Drechsler, Moritz Herrera, Michael A Liu, Jing Pees, Barbara Jarstorff, Johanna Czerwinski, Anna Lubbock, Francesca Angelidou, Georgia Salzer, Liesa Moors, Karlis Arturs Paczia, Nicole Shi, Yi-Ming Schulenburg, Hinrich Kaleta, Christoph Witting, Michael Liebeke, Manuel Campopiano, Dominic J Bode, Helge B Dierking, Katja Caenorhabditis elegans Animals Polyketide Synthases Sphingolipids Bacillus thuringiensis Pseudomonas fluorescens Gastrointestinal Microbiome Caenorhabditis elegans Proteins Serine C-Palmitoyltransferase Host-Pathogen Interactions Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components of host-microbiota interactions, their exact role in microbiota-mediated protection is largely unexplored. We addressed this knowledge gap with the nematode Caenorhabditis elegans and its microbiota isolate Pseudomonas fluorescens MYb115 that is known to protect against Bacillus thuringiensis (Bt) infection. We find that MYb115-mediated protection depends on sphingolipids (SLs) that are derived from an iterative type I polyketide synthase (PKS) cluster PfSgaAB, thereby revealing a non-canonical pathway for the production of bacterial SLs as secondary metabolites. SL production is common in eukaryotes but was thought to be limited to a few bacterial phyla that encode the serine palmitoyltransferase (SPT) enzyme, which catalyses the initial step in SL synthesis. We demonstrate that PfSgaB encodes a pyridoxal 5'-phosphate-dependent alpha-oxoamine synthase with SPT activity, and find homologous putative PKS clusters present across host-associated bacteria that are so far unknown SL producers. Moreover, we provide evidence that MYb115-derived SLs affect C. elegans defence against Bt infection by altering SL metabolism in the nematode host. This work establishes SLs as structural outputs of bacterial PKS and highlights the role of microbiota-derived SLs in host protection against pathogens. |
| title | Polyketide synthase-derived sphingolipids mediate microbiota protection against a bacterial pathogen in C. elegans. |
| topic | Caenorhabditis elegans Animals Polyketide Synthases Sphingolipids Bacillus thuringiensis Pseudomonas fluorescens Gastrointestinal Microbiome Caenorhabditis elegans Proteins Serine C-Palmitoyltransferase Host-Pathogen Interactions |
| url | https://pubmed.ncbi.nlm.nih.gov/40461452/ |