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| Autores principales: | , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
bioRxiv : the preprint server for biology
2025
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| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40475398/ |
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| _version_ | 1868266193703403522 |
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| author | Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton |
| author_facet | Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton |
| collection | PubMed - marine biology |
| contents | Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks. Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton Mollusks are an abundant group of animals with many economically important members that are phylogenetically distinct from nearly all genetic model organisms. This study provides clade-wide evaluation of sRNA biogenesis pathways, with emphasis on the easter oyster, . Understanding these molecules prescribes RNAi-based gene silencing approaches, benefiting genetic investigation and biotechnology. Similar to other animal groups, mollusks have conserved microRNAs (miRNAs) with some shared with ecdysozoans and deuterostomes; however, there was no evidence of an endogenous small-interfering RNA (siRNA) pathway. These results suggest that long double-stranded RNA (dsRNA)-based RNAi is not appropriate for gene silencing in Mollusks as well as other members of the broader Lophotrochozoan clade. The study also finds an abundance of piwi-interacting RNAs (piRNAs) in both soma and gonads. Differences are also found in piRNA biology. Many invertebrates exhibit somatic piRNAs; however, mollusk piRNAs appear to be restricted to a subset of cells, limiting the potential of piRNA-based RNAi. Further, individual animals also express a unique collection of piRNAs that seem to be only partially determined through inheritance from both parents. Together this work defines the RNAi mechanisms in mollusks, which represent 23% of animals, and provides insights into the phenotypic diversity seen in this group. |
| format | Artículo científico |
| id | pubmed_40475398 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks. Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks. Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton Mollusks are an abundant group of animals with many economically important members that are phylogenetically distinct from nearly all genetic model organisms. This study provides clade-wide evaluation of sRNA biogenesis pathways, with emphasis on the easter oyster, . Understanding these molecules prescribes RNAi-based gene silencing approaches, benefiting genetic investigation and biotechnology. Similar to other animal groups, mollusks have conserved microRNAs (miRNAs) with some shared with ecdysozoans and deuterostomes; however, there was no evidence of an endogenous small-interfering RNA (siRNA) pathway. These results suggest that long double-stranded RNA (dsRNA)-based RNAi is not appropriate for gene silencing in Mollusks as well as other members of the broader Lophotrochozoan clade. The study also finds an abundance of piwi-interacting RNAs (piRNAs) in both soma and gonads. Differences are also found in piRNA biology. Many invertebrates exhibit somatic piRNAs; however, mollusk piRNAs appear to be restricted to a subset of cells, limiting the potential of piRNA-based RNAi. Further, individual animals also express a unique collection of piRNAs that seem to be only partially determined through inheritance from both parents. Together this work defines the RNAi mechanisms in mollusks, which represent 23% of animals, and provides insights into the phenotypic diversity seen in this group. |
| title | Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks. |
| url | https://pubmed.ncbi.nlm.nih.gov/40475398/ |