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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40475398/ |
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Table of Contents:
- Implications of Endogenous Small Regulatory RNAs on Gene Silencing in Mollusks. Von Eiff, Cory Zancanela, Beatriz Schueng Gima, Megan Quito, Kevin Kotikalapudi, Manitejus Valdivia, Sergio Santos-Ortega, Yulica Flynt, Alex Sutton Mollusks are an abundant group of animals with many economically important members that are phylogenetically distinct from nearly all genetic model organisms. This study provides clade-wide evaluation of sRNA biogenesis pathways, with emphasis on the easter oyster, . Understanding these molecules prescribes RNAi-based gene silencing approaches, benefiting genetic investigation and biotechnology. Similar to other animal groups, mollusks have conserved microRNAs (miRNAs) with some shared with ecdysozoans and deuterostomes; however, there was no evidence of an endogenous small-interfering RNA (siRNA) pathway. These results suggest that long double-stranded RNA (dsRNA)-based RNAi is not appropriate for gene silencing in Mollusks as well as other members of the broader Lophotrochozoan clade. The study also finds an abundance of piwi-interacting RNAs (piRNAs) in both soma and gonads. Differences are also found in piRNA biology. Many invertebrates exhibit somatic piRNAs; however, mollusk piRNAs appear to be restricted to a subset of cells, limiting the potential of piRNA-based RNAi. Further, individual animals also express a unique collection of piRNAs that seem to be only partially determined through inheritance from both parents. Together this work defines the RNAi mechanisms in mollusks, which represent 23% of animals, and provides insights into the phenotypic diversity seen in this group.