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| Natura: | Artículo científico |
| Lingua: | en |
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Fish & shellfish immunology
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40482865/ |
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| _version_ | 1868266193692917760 |
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| author | Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua |
| author_facet | Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua |
| collection | PubMed - marine biology |
| contents | Grouper interleukin 15-like acts as a cytokine adjuvant in grouper iridovirus subunit vaccine. Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua Animals Fish Diseases Bass Vaccines, Subunit Viral Vaccines Ranavirus Fish Proteins Adjuvants, Immunologic DNA Virus Infections Interleukin-15 Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Gene Expression Regulation Gene Expression Profiling Interleukin (IL) 15 and IL-15-like (IL-15L) are members of the cytokine receptor γ chain (γc) family proteins, which play critical roles in the growth, and differentiation of various cell lineages of the innate and adaptive immune systems. However, the functions of teleost IL-15L upon virus infection still remained unknown. In the current study, a homolog of IL-15L from grouper (Epinephelus coioides) was identified and characterized. Sequence analysis indicated that EcIL-15L contained a conserved IL-15 domain (aa 13-134). Quantitative Reverse Transcription PCR (qRT-PCR) analysis indicated that the expression level of EcIL-15L was markedly increased upon Singapore grouper iridovirus (SGIV) infection, indicating that EcIL-15L may be involved in the host's defense against infections by fish viruses. In addition, EcIL-15L mostly colocalized with endoplasmic reticulum, and partially colocalized with the Golgi apparatus. EcIL-15L overexpression significantly decreased SGIV replication, as demonstrated by a reduce in viral gene transcriptions and protein synthesis. In contrast, the replication of SGIV was promoted by the knockdown of EcIL-15L in grouper cells. Furthermore, compared to SGIV subunit vaccine alone, the combination of vaccine and EcIL-15L significantly improved the relative protective effect against SGIV. Meanwhile, the combination of EcIL-15L significantly increased the specific antibody titer in serum or the transcription levels of immune related genes in vivo, respectively. In conclusion, our findings indicated that EcIL-15L exerted antiviral function against SGIV, and acted as a cytokine adjuvant to improve protective effects induced by SGIV subunit vaccine. |
| format | Artículo científico |
| id | pubmed_40482865 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Grouper interleukin 15-like acts as a cytokine adjuvant in grouper iridovirus subunit vaccine. Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua Animals Fish Diseases Bass Vaccines, Subunit Viral Vaccines Ranavirus Fish Proteins Adjuvants, Immunologic DNA Virus Infections Interleukin-15 Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Gene Expression Regulation Gene Expression Profiling Grouper interleukin 15-like acts as a cytokine adjuvant in grouper iridovirus subunit vaccine. Zheng, Jiaying Chen, Haotian Liu, Qiting Lin, Feifei Guo, Xixi Huang, Xiaohong Qin, Qiwei Huang, Youhua Animals Fish Diseases Bass Vaccines, Subunit Viral Vaccines Ranavirus Fish Proteins Adjuvants, Immunologic DNA Virus Infections Interleukin-15 Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Gene Expression Regulation Gene Expression Profiling Interleukin (IL) 15 and IL-15-like (IL-15L) are members of the cytokine receptor γ chain (γc) family proteins, which play critical roles in the growth, and differentiation of various cell lineages of the innate and adaptive immune systems. However, the functions of teleost IL-15L upon virus infection still remained unknown. In the current study, a homolog of IL-15L from grouper (Epinephelus coioides) was identified and characterized. Sequence analysis indicated that EcIL-15L contained a conserved IL-15 domain (aa 13-134). Quantitative Reverse Transcription PCR (qRT-PCR) analysis indicated that the expression level of EcIL-15L was markedly increased upon Singapore grouper iridovirus (SGIV) infection, indicating that EcIL-15L may be involved in the host's defense against infections by fish viruses. In addition, EcIL-15L mostly colocalized with endoplasmic reticulum, and partially colocalized with the Golgi apparatus. EcIL-15L overexpression significantly decreased SGIV replication, as demonstrated by a reduce in viral gene transcriptions and protein synthesis. In contrast, the replication of SGIV was promoted by the knockdown of EcIL-15L in grouper cells. Furthermore, compared to SGIV subunit vaccine alone, the combination of vaccine and EcIL-15L significantly improved the relative protective effect against SGIV. Meanwhile, the combination of EcIL-15L significantly increased the specific antibody titer in serum or the transcription levels of immune related genes in vivo, respectively. In conclusion, our findings indicated that EcIL-15L exerted antiviral function against SGIV, and acted as a cytokine adjuvant to improve protective effects induced by SGIV subunit vaccine. |
| title | Grouper interleukin 15-like acts as a cytokine adjuvant in grouper iridovirus subunit vaccine. |
| topic | Animals Fish Diseases Bass Vaccines, Subunit Viral Vaccines Ranavirus Fish Proteins Adjuvants, Immunologic DNA Virus Infections Interleukin-15 Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Gene Expression Regulation Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/40482865/ |