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Main Authors: Li, Chengcheng, Yue, Yufei, Yin, Rui, Zhao, Jiulong, Wang, Zengmeng, Nair, Shailesh, Zhang, Yongyu
Format: Artículo científico
Language:en
Published: Virology journal 2025
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Online Access:https://pubmed.ncbi.nlm.nih.gov/40483502/
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author Li, Chengcheng
Yue, Yufei
Yin, Rui
Zhao, Jiulong
Wang, Zengmeng
Nair, Shailesh
Zhang, Yongyu
author_facet Li, Chengcheng
Yue, Yufei
Yin, Rui
Zhao, Jiulong
Wang, Zengmeng
Nair, Shailesh
Zhang, Yongyu
Li, Chengcheng
Yue, Yufei
Yin, Rui
Zhao, Jiulong
Wang, Zengmeng
Nair, Shailesh
Zhang, Yongyu
collection PubMed - marine biology
contents Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture. Li, Chengcheng Yue, Yufei Yin, Rui Zhao, Jiulong Wang, Zengmeng Nair, Shailesh Zhang, Yongyu Shewanella Aquaculture Bacteriophages Host Specificity Phylogeny Genome, Viral Vibrio Proteomics Bacteriophages represent a promising alternative to antibiotics for controlling bacterial pathogens. However, phage application is often hindered by its narrow host range in preventing diseases caused by multiple unknown pathogens. While broad-host-range phages capable of cross-genus or cross-order infections, offer significant advantages in addressing this challenge, they are rarely isolated. In this study, we isolated two polyvalent lytic phages, SA-P and SA-M, through a multi-host enrichment strategy. These phages exhibited remarkable cross-order infectivity against the co-occurring aquaculture pathogens Shewanella algae and multiple Vibrio species. We confirmed that SA-P executes a complete lytic cycle in these cross-order hosts, indicating exceptional compatibility of its lysis systems across taxonomic orders. Genomic analysis revealed that their broad host recognition ability may stem from their diverse tail fiber and tailspike proteins. Notably, SA-P and SA-M are the first phages reported to infect S. algae, and their combined application exhibited a sustained suppression of pathogen growth. Proteomic phylogenetic analysis suggests these phages represent a novel unclassified viral genus and family, respectively. This study provides two promising polyvalent phages and their cocktails as potential solution for cross-order pathogen control in aquaculture.
format Artículo científico
id pubmed_40483502
institution PubMed
language en
publishDate 2025
publisher Virology journal
record_format pubmed
spellingShingle Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture.
Li, Chengcheng
Yue, Yufei
Yin, Rui
Zhao, Jiulong
Wang, Zengmeng
Nair, Shailesh
Zhang, Yongyu
Shewanella
Aquaculture
Bacteriophages
Host Specificity
Phylogeny
Genome, Viral
Vibrio
Proteomics
Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture. Li, Chengcheng Yue, Yufei Yin, Rui Zhao, Jiulong Wang, Zengmeng Nair, Shailesh Zhang, Yongyu Shewanella Aquaculture Bacteriophages Host Specificity Phylogeny Genome, Viral Vibrio Proteomics Bacteriophages represent a promising alternative to antibiotics for controlling bacterial pathogens. However, phage application is often hindered by its narrow host range in preventing diseases caused by multiple unknown pathogens. While broad-host-range phages capable of cross-genus or cross-order infections, offer significant advantages in addressing this challenge, they are rarely isolated. In this study, we isolated two polyvalent lytic phages, SA-P and SA-M, through a multi-host enrichment strategy. These phages exhibited remarkable cross-order infectivity against the co-occurring aquaculture pathogens Shewanella algae and multiple Vibrio species. We confirmed that SA-P executes a complete lytic cycle in these cross-order hosts, indicating exceptional compatibility of its lysis systems across taxonomic orders. Genomic analysis revealed that their broad host recognition ability may stem from their diverse tail fiber and tailspike proteins. Notably, SA-P and SA-M are the first phages reported to infect S. algae, and their combined application exhibited a sustained suppression of pathogen growth. Proteomic phylogenetic analysis suggests these phages represent a novel unclassified viral genus and family, respectively. This study provides two promising polyvalent phages and their cocktails as potential solution for cross-order pathogen control in aquaculture.
title Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture.
topic Shewanella
Aquaculture
Bacteriophages
Host Specificity
Phylogeny
Genome, Viral
Vibrio
Proteomics
url https://pubmed.ncbi.nlm.nih.gov/40483502/