Widespread distribution of bacteria containing PETases with a functional motif across global oceans.
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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The ISME journal
2025
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| author | Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M |
| author_facet | Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M |
| collection | PubMed - marine biology |
| contents | Widespread distribution of bacteria containing PETases with a functional motif across global oceans. Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M Bacteria Oceans and Seas Polyethylene Terephthalates Seawater Metagenomics Metagenome Computational Biology Phylogeny Accumulating evidence indicates that microorganisms respond to the ubiquitous plastic pollution by evolving plastic-degrading enzymes. However, the functional diversity of these enzymes and their distribution across the ocean, including the deep sea, remain poorly understood. By integrating bioinformatics and artificial intelligence-based structure prediction, we developed a structure- and function-informed algorithm to computationally distinguish functional polyethylene terephthalate-degrading enzymes (PETases) from variants lacking PETase activity (pseudo-PETase), either due to alternative substrate specificity or pseudogene origin. Through in vitro functional screening and in vivo microcosm experiments, we verified that this algorithm identified a high-confidence, searchable sequence motif for functional PETases capable of degrading PET. Metagenomic analysis of 415 ocean samples revealed 23 PETase variants, detected in nearly 80% of the samples. These PETases mainly occur between 1,000 and 2,000 m deep and at the surface in regions with high plastic pollution. Metatranscriptomic analysis further identified PETase variants that were actively transcribed by marine microorganisms. In contrast to their terrestrial counterparts-where PETases are taxonomically diverse-those in marine ecosystems were predominantly encoded and transcribed by members of the Pseudomonadales order. Our study underscores the widespread distribution of PETase-containing bacteria across carbon-limited marine ecosystems, identifying and distinguishing the PETase motif that underpins the functionality of these specialized cutinases. |
| format | Artículo científico |
| id | pubmed_40492703 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | The ISME journal |
| record_format | pubmed |
| spellingShingle | Widespread distribution of bacteria containing PETases with a functional motif across global oceans. Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M Bacteria Oceans and Seas Polyethylene Terephthalates Seawater Metagenomics Metagenome Computational Biology Phylogeny Widespread distribution of bacteria containing PETases with a functional motif across global oceans. Alam, Intikhab Marasco, Ramona Momin, Afaque A Aalismail, Nojood Laiolo, Elisa Martin, Cecilia Sanz-Sáez, Isabel Baltá Foix, Begoña Sá, Elisabet L Kamau, Allan Guzmán-Vega, Francisco J Jamil, Tahira Acinas, Silvia G Gasol, Josep M Gojobori, Takashi Agusti, Susana Daffonchio, Daniele Arold, Stefan T Duarte, Carlos M Bacteria Oceans and Seas Polyethylene Terephthalates Seawater Metagenomics Metagenome Computational Biology Phylogeny Accumulating evidence indicates that microorganisms respond to the ubiquitous plastic pollution by evolving plastic-degrading enzymes. However, the functional diversity of these enzymes and their distribution across the ocean, including the deep sea, remain poorly understood. By integrating bioinformatics and artificial intelligence-based structure prediction, we developed a structure- and function-informed algorithm to computationally distinguish functional polyethylene terephthalate-degrading enzymes (PETases) from variants lacking PETase activity (pseudo-PETase), either due to alternative substrate specificity or pseudogene origin. Through in vitro functional screening and in vivo microcosm experiments, we verified that this algorithm identified a high-confidence, searchable sequence motif for functional PETases capable of degrading PET. Metagenomic analysis of 415 ocean samples revealed 23 PETase variants, detected in nearly 80% of the samples. These PETases mainly occur between 1,000 and 2,000 m deep and at the surface in regions with high plastic pollution. Metatranscriptomic analysis further identified PETase variants that were actively transcribed by marine microorganisms. In contrast to their terrestrial counterparts-where PETases are taxonomically diverse-those in marine ecosystems were predominantly encoded and transcribed by members of the Pseudomonadales order. Our study underscores the widespread distribution of PETase-containing bacteria across carbon-limited marine ecosystems, identifying and distinguishing the PETase motif that underpins the functionality of these specialized cutinases. |
| title | Widespread distribution of bacteria containing PETases with a functional motif across global oceans. |
| topic | Bacteria Oceans and Seas Polyethylene Terephthalates Seawater Metagenomics Metagenome Computational Biology Phylogeny |
| url | https://pubmed.ncbi.nlm.nih.gov/40492703/ |