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| Autores principales: | , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Animals : an open access journal from MDPI
2025
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| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40509032/ |
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| _version_ | 1868266191346204672 |
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| author | Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo |
| author_facet | Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo |
| collection | PubMed - marine biology |
| contents | Acute Effect of Short-Term Benzocaine Anesthesia on the Skin Mucus Microbiome of Atlantic salmon (). Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo Routine aquaculture practices such as capture, transportation, and handling can disrupt the relationship between commensal and opportunistic bacteria in the fish skin microbiome. Anesthetic baths are a common welfare practice in aquaculture to reduce stress during handling. However, to date, no studies assessed the effect of anesthetics on bacterial communities in fish skin mucus. This study is the first to evaluate the influence of benzocaine, a widely used anesthetic, on the skin mucus bacterial microbiome of Atlantic salmon reared in a recirculating aquaculture system (RAS). Using Illumina high-throughput 16S rRNA gene sequencing, we found that bacterial richness and diversity were significantly reduced in skin mucus samples from fish with anesthesia (ANE) when compared with those without anesthesia (CTR). The predominant bacterial classes in both groups were Gammaproteobacteria (54.1-62.6%) and Betaproteobacteria (22.6-22.9%). However, significant dissimilarities in beta diversity were observed between the bacterial community structure of salmon skin mucus samples from ANE and CTR. These findings demonstrate that benzocaine exposure alters skin mucus microbiome of Atlantic salmon potentially leading to dysbiosis. This study also provides baseline information on the bacterial communities of Atlantic salmon skin mucus microbiome in an RAS. As no temporal resampling was performed, the duration and persistence of these changes remain unknown and warrant further investigation. |
| format | Artículo científico |
| id | pubmed_40509032 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Animals : an open access journal from MDPI |
| record_format | pubmed |
| spellingShingle | Acute Effect of Short-Term Benzocaine Anesthesia on the Skin Mucus Microbiome of Atlantic salmon (). Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo Acute Effect of Short-Term Benzocaine Anesthesia on the Skin Mucus Microbiome of Atlantic salmon (). Martins, Patrícia Pimentel, Tânia Ribeiro, Nuno Calado, Ricardo Routine aquaculture practices such as capture, transportation, and handling can disrupt the relationship between commensal and opportunistic bacteria in the fish skin microbiome. Anesthetic baths are a common welfare practice in aquaculture to reduce stress during handling. However, to date, no studies assessed the effect of anesthetics on bacterial communities in fish skin mucus. This study is the first to evaluate the influence of benzocaine, a widely used anesthetic, on the skin mucus bacterial microbiome of Atlantic salmon reared in a recirculating aquaculture system (RAS). Using Illumina high-throughput 16S rRNA gene sequencing, we found that bacterial richness and diversity were significantly reduced in skin mucus samples from fish with anesthesia (ANE) when compared with those without anesthesia (CTR). The predominant bacterial classes in both groups were Gammaproteobacteria (54.1-62.6%) and Betaproteobacteria (22.6-22.9%). However, significant dissimilarities in beta diversity were observed between the bacterial community structure of salmon skin mucus samples from ANE and CTR. These findings demonstrate that benzocaine exposure alters skin mucus microbiome of Atlantic salmon potentially leading to dysbiosis. This study also provides baseline information on the bacterial communities of Atlantic salmon skin mucus microbiome in an RAS. As no temporal resampling was performed, the duration and persistence of these changes remain unknown and warrant further investigation. |
| title | Acute Effect of Short-Term Benzocaine Anesthesia on the Skin Mucus Microbiome of Atlantic salmon (). |
| url | https://pubmed.ncbi.nlm.nih.gov/40509032/ |