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| Format: | Artículo científico |
| Language: | en |
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Luminescence : the journal of biological and chemical luminescence
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40524641/ |
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| author | Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana |
| author_facet | Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana |
| collection | PubMed - marine biology |
| contents | Anticancer Effects of Monacolin X Against Human Liver Cancer Cell Line: Exploring the Apoptosis Using AO/EB and DCFHDA Fluorescent Staining. Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana Humans Apoptosis Antineoplastic Agents Hep G2 Cells Liver Neoplasms Reactive Oxygen Species Drug Screening Assays, Antitumor Dose-Response Relationship, Drug Cell Proliferation Fluorescent Dyes Molecular Structure Hepatocellular carcinoma (HCC) most prevalent form of liver cancer and poses a few available treatments and is a major worldwide health burden. Monacolin X, a natural compound derived from the marine sponge-associated symbiont Monascus ruber, has garnered attention for its potential anticancer and anti-angiogenesis properties. This current study aimed to investigate the anticancer and apoptosis-inducing effects of Monacolin X against the human liver cancer (HepG2) cell line in vitro. This present study utilized various assays to assess cytotoxicity by MTT assay, apoptosis induction, DCFH-DA staining to measure the intracellular ROS levels, and apoptosis-and inflammation-related gene expression changes induced by the Monacolin X. The MTT results discovered dose-dependent cytotoxicity in HepG2 cells with an IC value of 72.4 μM. The apoptosis-inducing effect of Monacolin X was evident through propidium iodide (PI) and acridine orange/ethidium bromide (AO/EB) staining, accompanied by increased intracellular ROS levels and downregulated expression of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) and modulation of key apoptosis regulators (Bax and Bcl-2) as determined by qPCR analysis. In conclusion, these observations suggest a mechanism whereby Monacolin X has potent anticancer activity against the HepG2 cell line, and further investigation will be required to determine the molecular pathways responsible for the potential therapeutic effects for the clinical implications in liver cancer. |
| format | Artículo científico |
| id | pubmed_40524641 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Luminescence : the journal of biological and chemical luminescence |
| record_format | pubmed |
| spellingShingle | Anticancer Effects of Monacolin X Against Human Liver Cancer Cell Line: Exploring the Apoptosis Using AO/EB and DCFHDA Fluorescent Staining. Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana Humans Apoptosis Antineoplastic Agents Hep G2 Cells Liver Neoplasms Reactive Oxygen Species Drug Screening Assays, Antitumor Dose-Response Relationship, Drug Cell Proliferation Fluorescent Dyes Molecular Structure Anticancer Effects of Monacolin X Against Human Liver Cancer Cell Line: Exploring the Apoptosis Using AO/EB and DCFHDA Fluorescent Staining. Jayaraman, Vennila Arumugam, Madan Kumar Balachandran, Shana Boopathy, Lokeshkumar Arumugam, Sasikumar Srirangaramasamy, Jamunarani Devanesan, Sandhanasamy Suresh, Arumugam Sampath, Shobana Humans Apoptosis Antineoplastic Agents Hep G2 Cells Liver Neoplasms Reactive Oxygen Species Drug Screening Assays, Antitumor Dose-Response Relationship, Drug Cell Proliferation Fluorescent Dyes Molecular Structure Hepatocellular carcinoma (HCC) most prevalent form of liver cancer and poses a few available treatments and is a major worldwide health burden. Monacolin X, a natural compound derived from the marine sponge-associated symbiont Monascus ruber, has garnered attention for its potential anticancer and anti-angiogenesis properties. This current study aimed to investigate the anticancer and apoptosis-inducing effects of Monacolin X against the human liver cancer (HepG2) cell line in vitro. This present study utilized various assays to assess cytotoxicity by MTT assay, apoptosis induction, DCFH-DA staining to measure the intracellular ROS levels, and apoptosis-and inflammation-related gene expression changes induced by the Monacolin X. The MTT results discovered dose-dependent cytotoxicity in HepG2 cells with an IC value of 72.4 μM. The apoptosis-inducing effect of Monacolin X was evident through propidium iodide (PI) and acridine orange/ethidium bromide (AO/EB) staining, accompanied by increased intracellular ROS levels and downregulated expression of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) and modulation of key apoptosis regulators (Bax and Bcl-2) as determined by qPCR analysis. In conclusion, these observations suggest a mechanism whereby Monacolin X has potent anticancer activity against the HepG2 cell line, and further investigation will be required to determine the molecular pathways responsible for the potential therapeutic effects for the clinical implications in liver cancer. |
| title | Anticancer Effects of Monacolin X Against Human Liver Cancer Cell Line: Exploring the Apoptosis Using AO/EB and DCFHDA Fluorescent Staining. |
| topic | Humans Apoptosis Antineoplastic Agents Hep G2 Cells Liver Neoplasms Reactive Oxygen Species Drug Screening Assays, Antitumor Dose-Response Relationship, Drug Cell Proliferation Fluorescent Dyes Molecular Structure |
| url | https://pubmed.ncbi.nlm.nih.gov/40524641/ |