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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
BMC biotechnology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40537753/ |
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| _version_ | 1868266188993200128 |
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| author | Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S |
| author_facet | Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S |
| collection | PubMed - marine biology |
| contents | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells. Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S Humans Streptomyces Antineoplastic Agents MCF-7 Cells Breast Neoplasms Female Apoptosis Antioxidants Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity. |
| format | Artículo científico |
| id | pubmed_40537753 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | BMC biotechnology |
| record_format | pubmed |
| spellingShingle | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells. Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S Humans Streptomyces Antineoplastic Agents MCF-7 Cells Breast Neoplasms Female Apoptosis Antioxidants Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells. Shata, Abir M Rejili, Mokhtar Saeed El-Naggar, Manal M El-Asser, Samy A Saleh, Ahmed A Hafez, Elsayed E Haouala, Faouzi Youssef, Amany S Humans Streptomyces Antineoplastic Agents MCF-7 Cells Breast Neoplasms Female Apoptosis Antioxidants Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity. |
| title | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells. |
| topic | Humans Streptomyces Antineoplastic Agents MCF-7 Cells Breast Neoplasms Female Apoptosis Antioxidants |
| url | https://pubmed.ncbi.nlm.nih.gov/40537753/ |