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Main Authors:	Talice, Shani, Barkan, Shany K, Snyder, Grace A, Kozlovski, Itamar, Sharoni, Ton, Levy, Tom, Lewandowska, Magda, Ehrens, David, Sultan, Eliya, Azarov, Alona, Oisher, Shelly, Yannai, Ronnie, Aharoni, Reuven, Hadad, Uzi, Davis, Claytus, Moran, Yehu, Gershoni-Yahalom, Orly, Traylor-Knowles, Nikki, Rosental, Benyamin
Format:	Artículo científico
Language:	en
Published:	STAR protocols 2025
Subjects:	Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence
Online Access:	https://pubmed.ncbi.nlm.nih.gov/40543036/
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author	Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin
author_facet	Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin
collection	PubMed - marine biology
contents	Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al..
format	Artículo científico
id	pubmed_40543036
institution	PubMed
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publishDate	2025
publisher	STAR protocols
record_format	pubmed
spellingShingle	Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al..
title	Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model.
topic	Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence
url	https://pubmed.ncbi.nlm.nih.gov/40543036/

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