Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model.
Fuente:
PubMed
Salvato in:
| Autori principali: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
STAR protocols
2025
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266188983762944 |
|---|---|
| author | Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin |
| author_facet | Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin |
| collection | PubMed - marine biology |
| contents | Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.. |
| format | Artículo científico |
| id | pubmed_40543036 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | STAR protocols |
| record_format | pubmed |
| spellingShingle | Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. Talice, Shani Barkan, Shany K Snyder, Grace A Kozlovski, Itamar Sharoni, Ton Levy, Tom Lewandowska, Magda Ehrens, David Sultan, Eliya Azarov, Alona Oisher, Shelly Yannai, Ronnie Aharoni, Reuven Hadad, Uzi Davis, Claytus Moran, Yehu Gershoni-Yahalom, Orly Traylor-Knowles, Nikki Rosental, Benyamin Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.. |
| title | Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. |
| topic | Animals Flow Cytometry Sea Anemones Anthozoa Stem Cell Transplantation Microscopy, Fluorescence |
| url | https://pubmed.ncbi.nlm.nih.gov/40543036/ |