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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
ACS synthetic biology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40545707/ |
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Table of Contents:
- Engineered Marine Biofilms for Ocean Environment Monitoring. Nevot, Guillermo Pol Cros, Maria Toloza, Lorena Campamà-Sanz, Nil Artigues-Lleixà, Maria Aguilera, Laura Güell, Marc Biofilms Biosensing Techniques Environmental Monitoring Oceans and Seas Monophenol Monooxygenase Bacterial Proteins Marine bacteria offer a promising alternative for developing Engineered Living Materials (ELMs) tailored to marine applications. We engineered to increase its surface-associated growth and develop biosensors for ocean environment monitoring. By fusing the endogenous extracellular matrix amyloidogenic protein CsgA with mussel foot proteins, we significantly increased biofilm formation. Additionally, was engineered to express the tyrosinase enzyme to further enhance microbial attachment through post-translational modifications of tyrosine residues. By exploiting natural genetic resources, two environmental biosensors were created to detect temperature and oxygen. These biosensors were coupled with a CRISPR-based recording system to store transient gene expression in stable DNA arrays, enabling long-term environmental monitoring. These engineered strains highlight potential in advancing marine microbiome engineering for innovative biofilm applications, including the development of natural, self-renewing biological adhesives, environmental sensors, and "sentinel" cells equipped with CRISPR-recording technology to capture and store environmental signals.