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Autores principales: Kakar, Rozina, Anis, Siti Nor Syairah, Vigneswari, Sevakumaran, Abdullah, Amirul Al-Ashraf
Formato: Artículo científico
Lenguaje:en
Publicado: Biotechnology and applied biochemistry 2026
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Acceso en línea:https://pubmed.ncbi.nlm.nih.gov/40551550/
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author Kakar, Rozina
Anis, Siti Nor Syairah
Vigneswari, Sevakumaran
Abdullah, Amirul Al-Ashraf
author_facet Kakar, Rozina
Anis, Siti Nor Syairah
Vigneswari, Sevakumaran
Abdullah, Amirul Al-Ashraf
Kakar, Rozina
Anis, Siti Nor Syairah
Vigneswari, Sevakumaran
Abdullah, Amirul Al-Ashraf
collection PubMed - marine biology
contents Valorization of Biodiesel Waste, Glycerine Pitch as a Substrate for Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Biosynthesis. Kakar, Rozina Anis, Siti Nor Syairah Vigneswari, Sevakumaran Abdullah, Amirul Al-Ashraf Polyesters Glycerol Biofuels Cupriavidus Polyhydroxybutyrates Studies that focus on turning biodiesel byproducts into valuable products have recently garnered increasing attention. This investigation highlights the utilization of waste substrates for a circular economy approach using glycerine pitch as the main carbon source to produce biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [(P(3HB-co-3HV)] by Cupriavidus malaysiensis USMAA1020. The primary use of glycerine pitch, along with several parameters that may affect the growth and biosynthesis of copolymers, are investigated. The utilization of glycerine pitch along with 1-pentanol and oleic acid has the most effective effect on bacterial growth and copolymer accumulation. Response surface methodology (RSM) was used to optimize these variables. The polyhydroxyalkanoate (PHA) content increased up to 77.7 wt% from 68.9 wt%, with residual dry cell weight (RDCW) of 6.5 g/L from 4.5 g/L. The optimal conditions for producing various compositions of the 3HVs were also determined. The three selected copolymer compositions were P(3HB-co-4%3HV), P(3HB-co-11%3HV), and P(3HB-co-18%3HV). Moreover, varying the copolymer compositions produced distinct polymer characteristics. According to this study, P(3HB-co-3HV) with variable properties can be produced for a range of applications using glycerine pitch as a potential primary carbon source. In addition to reducing the cost of production, this would enhance efficient waste management.
format Artículo científico
id pubmed_40551550
institution PubMed
language en
publishDate 2026
publisher Biotechnology and applied biochemistry
record_format pubmed
spellingShingle Valorization of Biodiesel Waste, Glycerine Pitch as a Substrate for Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Biosynthesis.
Kakar, Rozina
Anis, Siti Nor Syairah
Vigneswari, Sevakumaran
Abdullah, Amirul Al-Ashraf
Polyesters
Glycerol
Biofuels
Cupriavidus
Polyhydroxybutyrates
Valorization of Biodiesel Waste, Glycerine Pitch as a Substrate for Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Biosynthesis. Kakar, Rozina Anis, Siti Nor Syairah Vigneswari, Sevakumaran Abdullah, Amirul Al-Ashraf Polyesters Glycerol Biofuels Cupriavidus Polyhydroxybutyrates Studies that focus on turning biodiesel byproducts into valuable products have recently garnered increasing attention. This investigation highlights the utilization of waste substrates for a circular economy approach using glycerine pitch as the main carbon source to produce biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [(P(3HB-co-3HV)] by Cupriavidus malaysiensis USMAA1020. The primary use of glycerine pitch, along with several parameters that may affect the growth and biosynthesis of copolymers, are investigated. The utilization of glycerine pitch along with 1-pentanol and oleic acid has the most effective effect on bacterial growth and copolymer accumulation. Response surface methodology (RSM) was used to optimize these variables. The polyhydroxyalkanoate (PHA) content increased up to 77.7 wt% from 68.9 wt%, with residual dry cell weight (RDCW) of 6.5 g/L from 4.5 g/L. The optimal conditions for producing various compositions of the 3HVs were also determined. The three selected copolymer compositions were P(3HB-co-4%3HV), P(3HB-co-11%3HV), and P(3HB-co-18%3HV). Moreover, varying the copolymer compositions produced distinct polymer characteristics. According to this study, P(3HB-co-3HV) with variable properties can be produced for a range of applications using glycerine pitch as a potential primary carbon source. In addition to reducing the cost of production, this would enhance efficient waste management.
title Valorization of Biodiesel Waste, Glycerine Pitch as a Substrate for Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Biosynthesis.
topic Polyesters
Glycerol
Biofuels
Cupriavidus
Polyhydroxybutyrates
url https://pubmed.ncbi.nlm.nih.gov/40551550/