Housing matters: Experimental variables shaping metabolism in obese mice.
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| Format: | Artículo científico |
| Sprache: | en |
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Molecular metabolism
2025
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| author | Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H |
| author_facet | Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H |
| collection | PubMed - marine biology |
| contents | Housing matters: Experimental variables shaping metabolism in obese mice. Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H Animals Mice Male Obesity Female Mice, Inbred C57BL Housing, Animal Energy Metabolism Mice, Obese Weight Gain Glucose Intolerance Liver Diet-induced obesity in mice is an important model for investigating host-diet interactions as well as dietary and pharmacological treatments of metabolic diseases. Experimental reproducibility is, however, a recurrent challenge. To determine key controllable experimental drivers of mouse metabolism, we distributed 338C57BL/6JBomTac mice (males and females) into six research units across two countries, divided them into a variety of housing conditions (i.e., diets, cage types, temperatures, group-housing vs. single-housing) and kept 26 reference mice at the vendor. We applied linear mixed models to rank the influence of each variable on metabolic phenotype (i.e., body weight gain, glucose intolerance, liver, and visceral adipose tissue weight). Group-housing was the most potent driver of metabolic dysfunction apart from sex and diet. Accordingly, single-housed mice exhibited reduced weight gain (∼50%), increased energy expenditure, and diminished respiratory exchange ratio concomitant with improved glucose tolerance (∼20%) compared to their group-housed counterparts. Our results may aid in clarifying the impact of experimental design and promote rational, transparent reporting to increase reproducibility. |
| format | Artículo científico |
| id | pubmed_40554092 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Molecular metabolism |
| record_format | pubmed |
| spellingShingle | Housing matters: Experimental variables shaping metabolism in obese mice. Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H Animals Mice Male Obesity Female Mice, Inbred C57BL Housing, Animal Energy Metabolism Mice, Obese Weight Gain Glucose Intolerance Liver Housing matters: Experimental variables shaping metabolism in obese mice. Choi, Béatrice So-Yun Holm, Jacob Bak Brejnrod, Asker Fjære, Even Xia, Zhongkui Allingbjerg, Marie-Louise Larsen, Ida Søgaard Kristensen, David Møbjerg Dall, Morten Myrmel, Lene Secher Koch, Janne Danneskiold-Samsøe, Niels Banhos Kalliokoski, Otto Treebak, Jonas T Xiao, Liang Hansen, Axel Kornerup Sørensen, Helle Madsen, Lise Arumugam, Manimozhiyan Kristiansen, Karsten Jensen, Benjamin A H Animals Mice Male Obesity Female Mice, Inbred C57BL Housing, Animal Energy Metabolism Mice, Obese Weight Gain Glucose Intolerance Liver Diet-induced obesity in mice is an important model for investigating host-diet interactions as well as dietary and pharmacological treatments of metabolic diseases. Experimental reproducibility is, however, a recurrent challenge. To determine key controllable experimental drivers of mouse metabolism, we distributed 338C57BL/6JBomTac mice (males and females) into six research units across two countries, divided them into a variety of housing conditions (i.e., diets, cage types, temperatures, group-housing vs. single-housing) and kept 26 reference mice at the vendor. We applied linear mixed models to rank the influence of each variable on metabolic phenotype (i.e., body weight gain, glucose intolerance, liver, and visceral adipose tissue weight). Group-housing was the most potent driver of metabolic dysfunction apart from sex and diet. Accordingly, single-housed mice exhibited reduced weight gain (∼50%), increased energy expenditure, and diminished respiratory exchange ratio concomitant with improved glucose tolerance (∼20%) compared to their group-housed counterparts. Our results may aid in clarifying the impact of experimental design and promote rational, transparent reporting to increase reproducibility. |
| title | Housing matters: Experimental variables shaping metabolism in obese mice. |
| topic | Animals Mice Male Obesity Female Mice, Inbred C57BL Housing, Animal Energy Metabolism Mice, Obese Weight Gain Glucose Intolerance Liver |
| url | https://pubmed.ncbi.nlm.nih.gov/40554092/ |