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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Frontiers in microbiology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40556897/ |
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| _version_ | 1868266186747150336 |
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| author | Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan |
| author_facet | Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan |
| collection | PubMed - marine biology |
| contents | Two novel phages infecting isolated from the epipelagic ocean. Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan , an aerobic anoxygenic photoheterotrophic bacterial genus, plays a vital role in carbon and energy cycling in marine environments. However, their phage predators remain poorly understood, with only two strains previously reported. This study isolated and characterized a novel phage, vB_EauS-R34L1 (R34L1), and its sub-strain vB_EauS-R34L2 (R34L2), from coastal seawater. Both phages exhibit long-tailed, icosahedral morphologies and relatively narrow but slightly different host ranges. One-step growth curve analysis revealed a 160-min latent period and burst sizes of 81 and 91 PFU/cell for R34L1 and R34L2, respectively. Genomic analysis showed that the phages possess dsDNA genomes of 56,415 bp (R34L1) and 54,924 bp (R34L2), with G + C contents of 61.60 and 61.19%, respectively. Both phages harbor a suite of unique genes, including GapR and GH19, which are crucial for host interaction and ecological functionality. Blastn analysis indicated a 99.73% genome similarity between them. Taxonomic and phylogenetic analyses positioned them in a novel viral genus cluster, , under the family , indicating a distant evolutionary relationship with known phages. Metagenomic queries suggested that R34L1- and R34L2-like phages are exclusively abundant in temperate and tropical epipelagic zones. This study expands our understanding of phages and provides insights into their ecological roles in marine ecosystems. |
| format | Artículo científico |
| id | pubmed_40556897 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Frontiers in microbiology |
| record_format | pubmed |
| spellingShingle | Two novel phages infecting isolated from the epipelagic ocean. Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan Two novel phages infecting isolated from the epipelagic ocean. Lu, Longfei Huang, Xingyu Zheng, Pengfei Wei, Shuzhen Jiao, Nianzhi Zhang, Rui Li, Xuejing Yang, Yunlan , an aerobic anoxygenic photoheterotrophic bacterial genus, plays a vital role in carbon and energy cycling in marine environments. However, their phage predators remain poorly understood, with only two strains previously reported. This study isolated and characterized a novel phage, vB_EauS-R34L1 (R34L1), and its sub-strain vB_EauS-R34L2 (R34L2), from coastal seawater. Both phages exhibit long-tailed, icosahedral morphologies and relatively narrow but slightly different host ranges. One-step growth curve analysis revealed a 160-min latent period and burst sizes of 81 and 91 PFU/cell for R34L1 and R34L2, respectively. Genomic analysis showed that the phages possess dsDNA genomes of 56,415 bp (R34L1) and 54,924 bp (R34L2), with G + C contents of 61.60 and 61.19%, respectively. Both phages harbor a suite of unique genes, including GapR and GH19, which are crucial for host interaction and ecological functionality. Blastn analysis indicated a 99.73% genome similarity between them. Taxonomic and phylogenetic analyses positioned them in a novel viral genus cluster, , under the family , indicating a distant evolutionary relationship with known phages. Metagenomic queries suggested that R34L1- and R34L2-like phages are exclusively abundant in temperate and tropical epipelagic zones. This study expands our understanding of phages and provides insights into their ecological roles in marine ecosystems. |
| title | Two novel phages infecting isolated from the epipelagic ocean. |
| url | https://pubmed.ncbi.nlm.nih.gov/40556897/ |