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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Medical oncology (Northwood, London, England)
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40560420/ |
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Table of Contents:
- Potential anticancer properties of Tetraselmis suecica extract against oral and colorectal cancer cells. Asoudeh-Fard, Abbas Soltanmohammadi, Fatemeh Kashkouie Jahromi, Maryam Javanmardi, Fatemeh Roosta, Anis Zareian Jahromi, Mohammad Parsaei, Asghar Humans Colorectal Neoplasms Apoptosis Plant Extracts Mouth Neoplasms Human Umbilical Vein Endothelial Cells HT29 Cells Cell Survival Antineoplastic Agents Cell Line, Tumor Cell lines following treatment with the extract at their respective IC₅₀ concentrations (35 µg/ml for HT-29 and 20 µg/ml for KB). Gene expression analysis showed upregulation of pro- Background: Natural products are a rich source of anticancer agents. Tetraselmis suecica, a green microalga, has demonstrated potential anticancer effects in limited studies, though its impact on colorectal and oral cancers remains unexplored. This study aims to investigate the anticancer and pro-apoptotic effects of Tetraselmis suecica extract on human colorectal adenocarcinoma cells (HT-29) and oral epidermal carcinoma cells (KB), and to evaluate its safety on normal human umbilical vein endothelial cells (HUVEC). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to assess cell viability in HT-29, KB, and HUVEC cells. Apoptosis was evaluated using Annexin V/propidium iodide (PI) staining and flow cytometry. Real-time polymerase chain reaction (PCR) was performed to analyze the expression of apoptosis-related genes including Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, PTEN, and AKT. Tetraselmis suecica extract reduced cell viability in HT-29 and KB cells in a dose-dependent manner, with IC₅₀ values of approximately 35 µg/ml for HT-29 and 20 µg/ml for KB cells, as calculated from dose-response curves. The extract showed minimal cytotoxicity on HUVEC cells, maintaining over 95% viability even at the highest tested concentration. Apoptosis was significantly increased in both cancer apoptotic genes (Caspases, Bax, PTEN) and downregulation of anti-apoptotic genes (Bcl-2, AKT). This study is the first to report the pro-apoptotic and anticancer effects of Tetraselmis suecica extract on oral and colorectal cancer cells. The findings suggest its potential as a natural therapeutic agent, with minimal cytotoxicity on normal endothelial cells.