Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway.
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PubMed
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| Auteurs principaux: | , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
eLife
2025
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| _version_ | 1868266186736664577 |
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| author | Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong |
| author_facet | Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong |
| collection | PubMed - marine biology |
| contents | Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway. Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong Animals Pinocytosis Virus Internalization Nodaviridae Myosin Light Chains Oryzias RNA Virus Infections Fish Diseases Receptors, Virus Nodaviridae infections cause severe mortality in insects and fish, with nervous necrosis virus (NNV) posing significant threats to global fish populations. However, the host factors involved in NNV entry remain poorly understood. We identify myosin light chain 3 from marine medaka () (MmMYL3) as a novel receptor for red-spotted grouper NNV (RGNNV), facilitating internalization via macropinocytosis. MmMYL3 directly binds the RGNNV capsid protein (CP), which depends on the arm and S domains of CP and the EF-hand2 domain of MmMYL3. In vitro experiments showed that MmMYL3 siRNA, protein, anti-MYL3 antibodies, or the arm domain synthetic peptides blocked RGNNV entry. Moreover, in vivo administration of MmMYL3 protein also inhibited RGNNV infection. Ectopic MmMYL3 expression enabled RGNNV internalization into resistant cells. Notably, MmMYL3 facilitated RGNNV internalization through the macropinocytosis pathway via the IGF1R-Rac1/Cdc42 axis. Collectively, our findings underscore MYL3's crucial role in NNV entry and its potential as an antiviral target. |
| format | Artículo científico |
| id | pubmed_40560751 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | eLife |
| record_format | pubmed |
| spellingShingle | Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway. Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong Animals Pinocytosis Virus Internalization Nodaviridae Myosin Light Chains Oryzias RNA Virus Infections Fish Diseases Receptors, Virus Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway. Yao, Lan Zhang, Wanwan Yang, Xiaogang Yi, Meisheng Jia, Kuntong Animals Pinocytosis Virus Internalization Nodaviridae Myosin Light Chains Oryzias RNA Virus Infections Fish Diseases Receptors, Virus Nodaviridae infections cause severe mortality in insects and fish, with nervous necrosis virus (NNV) posing significant threats to global fish populations. However, the host factors involved in NNV entry remain poorly understood. We identify myosin light chain 3 from marine medaka () (MmMYL3) as a novel receptor for red-spotted grouper NNV (RGNNV), facilitating internalization via macropinocytosis. MmMYL3 directly binds the RGNNV capsid protein (CP), which depends on the arm and S domains of CP and the EF-hand2 domain of MmMYL3. In vitro experiments showed that MmMYL3 siRNA, protein, anti-MYL3 antibodies, or the arm domain synthetic peptides blocked RGNNV entry. Moreover, in vivo administration of MmMYL3 protein also inhibited RGNNV infection. Ectopic MmMYL3 expression enabled RGNNV internalization into resistant cells. Notably, MmMYL3 facilitated RGNNV internalization through the macropinocytosis pathway via the IGF1R-Rac1/Cdc42 axis. Collectively, our findings underscore MYL3's crucial role in NNV entry and its potential as an antiviral target. |
| title | Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway. |
| topic | Animals Pinocytosis Virus Internalization Nodaviridae Myosin Light Chains Oryzias RNA Virus Infections Fish Diseases Receptors, Virus |
| url | https://pubmed.ncbi.nlm.nih.gov/40560751/ |