Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights.
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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Water research
2025
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| _version_ | 1868266186731421696 |
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| author | Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang |
| author_facet | Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang |
| collection | PubMed - marine biology |
| contents | Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights. Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang Microbiota Halogenation Seawater Genomics Biodegradation, Environmental Geologic Sediments Deep-sea cold seeps harbor a rich and diverse repertoire of reductive dehalogenase-encoding genes (rdhA), yet their potential for reductive dehalogenation remains largely unexplored. In this study, we investigated the microbial debromination of 2,4,6-tribromophenol (TBP) in cold seep sediment microcosms. By optimizing culture conditions with different nutrient sources and substrate concentrations, we established a highly efficient debrominating microbial consortium capable of completely degrading 50 μM TBP within 72 h. Metagenomic analysis revealed Bin3, a novel bacterium affiliated with Peptococcaceae, as a key dehalogenator harboring multiple rdhA genes. Microbial community analysis demonstrated that nutrient availability significantly influenced beta diversity (community composition) but had only a minor effect on alpha diversity. Through degradation kinetics, co-occurrence network analysis, normalized stochasticity ratio analysis, and metagenomic quantification, we found that supplementing lactate along with 0.05 % yeast extract significantly enhanced TBP degradation efficiency and facilitated the targeted enrichment of key dehalogenating microbes (with relative abundance increasing from |
| format | Artículo científico |
| id | pubmed_40561629 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Water research |
| record_format | pubmed |
| spellingShingle | Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights. Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang Microbiota Halogenation Seawater Genomics Biodegradation, Environmental Geologic Sediments Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights. Deng, Zhaochao Xie, Yeting Yu, Hao Zhang, Xiaoyan Tan, Tianyang Kuang, Wenzhen Han, Zhiqiang Li, Yanhong Wang, Heng Zhang, Ning Zhang, Chunfang Microbiota Halogenation Seawater Genomics Biodegradation, Environmental Geologic Sediments Deep-sea cold seeps harbor a rich and diverse repertoire of reductive dehalogenase-encoding genes (rdhA), yet their potential for reductive dehalogenation remains largely unexplored. In this study, we investigated the microbial debromination of 2,4,6-tribromophenol (TBP) in cold seep sediment microcosms. By optimizing culture conditions with different nutrient sources and substrate concentrations, we established a highly efficient debrominating microbial consortium capable of completely degrading 50 μM TBP within 72 h. Metagenomic analysis revealed Bin3, a novel bacterium affiliated with Peptococcaceae, as a key dehalogenator harboring multiple rdhA genes. Microbial community analysis demonstrated that nutrient availability significantly influenced beta diversity (community composition) but had only a minor effect on alpha diversity. Through degradation kinetics, co-occurrence network analysis, normalized stochasticity ratio analysis, and metagenomic quantification, we found that supplementing lactate along with 0.05 % yeast extract significantly enhanced TBP degradation efficiency and facilitated the targeted enrichment of key dehalogenating microbes (with relative abundance increasing from |
| title | Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights. |
| topic | Microbiota Halogenation Seawater Genomics Biodegradation, Environmental Geologic Sediments |
| url | https://pubmed.ncbi.nlm.nih.gov/40561629/ |