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Main Authors:	Yoshida, Mitsuhiro, Medvedeva, Sofia, Fukudome, Akihito, Wolf, Yuri I, Urayama, Syun-Ichi, Nishimura, Yosuke, Takaki, Yoshihiro, Koonin, Eugene V, Krupovic, Mart, Nunoura, Takuro
Format:	Artículo científico
Language:	en
Published:	bioRxiv : the preprint server for biology 2025
Online Access:	https://pubmed.ncbi.nlm.nih.gov/40568107/
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author	Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro
author_facet	Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro
collection	PubMed - marine biology
contents	"Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes. Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro Fragmented and primer Ligated DsRNA Sequencing (FLDS) was used to reconstruct five complete, bisegmented RNA genomes of paraxenoviruses, a group of viruses that was previously identified in the ocean and that based on the analysis of partial genomes was proposed to represent a putative new phylum within the kingdom of the realm . Phylogenetic analysis of the RNA-directed RNA polymerases of paraxenoviruses demonstrated their affinity with the ribovirus order within the class of the phylum . The order includes families that consists of well-characterized dsRNA bacteriophages and less thoroughly studied that are also suspected to infect bacteria. Consistently, modeling and analysis of the structure of the predicted capsid protein (CP) of several paraxenoviruses revealed similarity to picobirnavirus CP although the paraxenovirus CP is much larger and contains unique structural elaborations. Taken together, these affinities suggest that paraxenoviruses represent a distinct family within , which we provisionally name "Paraxenoviridae". Both genomic segments in and "Paraxenoviridae" encompass multiple open reading frames, each preceded by a typical bacterial ribosome-binding site, strongly suggesting that these families consist of bacterial viruses. Search for homologs of paraxenovirus genes shows widespread distribution of this virus group in the global ocean, suggesting a potential important contribution to marine microbial ecosystems. Our findings further expand the diversity and ecological role of the bacterial RNA virome, reveal extensive structural variability of RNA viral capsid proteins, and demonstrate the common ancestry of several distinct families of bacterial viruses with dsRNA genomes.
format	Artículo científico
id	pubmed_40568107
institution	PubMed
language	en
publishDate	2025
publisher	bioRxiv : the preprint server for biology
record_format	pubmed
spellingShingle	"Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes. Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro "Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes. Yoshida, Mitsuhiro Medvedeva, Sofia Fukudome, Akihito Wolf, Yuri I Urayama, Syun-Ichi Nishimura, Yosuke Takaki, Yoshihiro Koonin, Eugene V Krupovic, Mart Nunoura, Takuro Fragmented and primer Ligated DsRNA Sequencing (FLDS) was used to reconstruct five complete, bisegmented RNA genomes of paraxenoviruses, a group of viruses that was previously identified in the ocean and that based on the analysis of partial genomes was proposed to represent a putative new phylum within the kingdom of the realm . Phylogenetic analysis of the RNA-directed RNA polymerases of paraxenoviruses demonstrated their affinity with the ribovirus order within the class of the phylum . The order includes families that consists of well-characterized dsRNA bacteriophages and less thoroughly studied that are also suspected to infect bacteria. Consistently, modeling and analysis of the structure of the predicted capsid protein (CP) of several paraxenoviruses revealed similarity to picobirnavirus CP although the paraxenovirus CP is much larger and contains unique structural elaborations. Taken together, these affinities suggest that paraxenoviruses represent a distinct family within , which we provisionally name "Paraxenoviridae". Both genomic segments in and "Paraxenoviridae" encompass multiple open reading frames, each preceded by a typical bacterial ribosome-binding site, strongly suggesting that these families consist of bacterial viruses. Search for homologs of paraxenovirus genes shows widespread distribution of this virus group in the global ocean, suggesting a potential important contribution to marine microbial ecosystems. Our findings further expand the diversity and ecological role of the bacterial RNA virome, reveal extensive structural variability of RNA viral capsid proteins, and demonstrate the common ancestry of several distinct families of bacterial viruses with dsRNA genomes.
title	"Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes.
url	https://pubmed.ncbi.nlm.nih.gov/40568107/

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