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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Journal of water and health
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40586335/ |
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| _version_ | 1868266184100544512 |
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| author | do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish |
| author_facet | do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish |
| collection | PubMed - marine biology |
| contents | Comparative evaluation of heating instruments for detecting HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP. do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish Wastewater SARS-CoV-2 Nucleic Acid Amplification Techniques Colorimetry Bacteroides Kobuvirus Molecular Diagnostic Techniques This study assessed the accuracy, field suitability, and cost-effectiveness of colorimetric LAMP (c-LAMP) assays using six heating instruments: Electric Kettle, Water Bath, Heating Block, miniPCR, Thermocycler, and Genie® III. Bacteroides HF183 (HF183), SARS-CoV-2, and Aichi virus A (AiV-A) were tested using control materials and wastewater samples, comparing cLAMP with qPCR/RT-qPCR detection. qPCR/RT-qPCR reliably detected HF183 and AiV-A genetic fragments at dilutions as low as 1 × 10 pg/μL, corresponding to 1.24 and 1.49 log GC/reaction. cLAMP/RT-cLAMP detected these fragments at 1 × 10 pg/μL (2.28 and 2.63 log GC/reaction), though Electric Kettle, miniPCR, and Genie® III occasionally produced false negatives. RT-qPCR reliably detected SARS-CoV-2 fragments at 1 × 10 GC/μL (1.86 log GC/reaction), with cLAMP/RT-cLAMP performing similarly across most instruments except Thermocycler. No significant differences (p > 0.05) were observed among instruments, though inconsistencies appeared at lower concentrations. The findings offer valuable insights into heating instrument performance for cLAMP/RT-cLAMP assays, guiding their application in wastewater-based pathogen detection. |
| format | Artículo científico |
| id | pubmed_40586335 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of water and health |
| record_format | pubmed |
| spellingShingle | Comparative evaluation of heating instruments for detecting HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP. do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish Wastewater SARS-CoV-2 Nucleic Acid Amplification Techniques Colorimetry Bacteroides Kobuvirus Molecular Diagnostic Techniques Comparative evaluation of heating instruments for detecting HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP. do Nascimento, Mariah C A Smith, Wendy J M Liu, Yawen da Costa, Vivaldo Gomes Simpson, Stuart L Bivins, Aaron Rahal, Paula Ahmed, Warish Wastewater SARS-CoV-2 Nucleic Acid Amplification Techniques Colorimetry Bacteroides Kobuvirus Molecular Diagnostic Techniques This study assessed the accuracy, field suitability, and cost-effectiveness of colorimetric LAMP (c-LAMP) assays using six heating instruments: Electric Kettle, Water Bath, Heating Block, miniPCR, Thermocycler, and Genie® III. Bacteroides HF183 (HF183), SARS-CoV-2, and Aichi virus A (AiV-A) were tested using control materials and wastewater samples, comparing cLAMP with qPCR/RT-qPCR detection. qPCR/RT-qPCR reliably detected HF183 and AiV-A genetic fragments at dilutions as low as 1 × 10 pg/μL, corresponding to 1.24 and 1.49 log GC/reaction. cLAMP/RT-cLAMP detected these fragments at 1 × 10 pg/μL (2.28 and 2.63 log GC/reaction), though Electric Kettle, miniPCR, and Genie® III occasionally produced false negatives. RT-qPCR reliably detected SARS-CoV-2 fragments at 1 × 10 GC/μL (1.86 log GC/reaction), with cLAMP/RT-cLAMP performing similarly across most instruments except Thermocycler. No significant differences (p > 0.05) were observed among instruments, though inconsistencies appeared at lower concentrations. The findings offer valuable insights into heating instrument performance for cLAMP/RT-cLAMP assays, guiding their application in wastewater-based pathogen detection. |
| title | Comparative evaluation of heating instruments for detecting HF183, SARS-CoV-2, and Aichi virus A in untreated wastewater using colorimetric LAMP. |
| topic | Wastewater SARS-CoV-2 Nucleic Acid Amplification Techniques Colorimetry Bacteroides Kobuvirus Molecular Diagnostic Techniques |
| url | https://pubmed.ncbi.nlm.nih.gov/40586335/ |