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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Biosensors & bioelectronics
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40592263/ |
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| _version_ | 1868266184093204480 |
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| author | Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen |
| author_facet | Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen |
| collection | PubMed - marine biology |
| contents | Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples. Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen Biosensing Techniques Sulfides Agrobacterium tumefaciens Geologic Sediments Bacterial Proteins Environmental Monitoring Supersulfides are a class of sulfur compounds in either zero-valent or monovalent negative states, such as elemental sulfur, inorganic polysulfides, and organic polysulfides, and they play crucial roles in biological systems, environmental sulfur cycling, and Li-S batteries. However, accurate quantification of these compounds remains challenging due to their reactive nature and structural complexity. Here, we report the first whole-cell biosensor for the specific detection of supersulfides using the supersulfide-sensing repressor BigR of Agrobacterium tumefaciens (AtBigR). This novel biosensor system shows exceptional selectivity for supersulfides with negligible response to HS, thiosulfate, and glutathione. Through systematic optimization of the AtBigR-based whole-cell biosensor (AtBigR-WCB), we developed a biosensor with negligible background leakage and high sensitivity, capable of detecting supersulfides at micromolar levels. We successfully applied AtBigR-WCB to quantify supersulfide contents in marine sediments from diverse seafloor topographies and lithium polysulfides in lithium-sulfur battery electrolytes. Widespread supersulfide accumulation was detected in deeper layers of marine sediments across various environments. Additionally, the optimized AtBigR regulatory system was a novel, low-leakage regulating system, which offers a new type of gene-regulating system with potential applications in synthetic biology. Our work establishes a new platform for quantifying supersulfides, which is helpful to further our understanding of supersulfides in biology, environments, and lithium sulfur batteries. |
| format | Artículo científico |
| id | pubmed_40592263 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biosensors & bioelectronics |
| record_format | pubmed |
| spellingShingle | Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples. Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen Biosensing Techniques Sulfides Agrobacterium tumefaciens Geologic Sediments Bacterial Proteins Environmental Monitoring Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples. Liu, Fan Wang, Min Liu, Huaiwei Gong, Xianzhe Du, Ning Qin, Qilong Tian, Yun Xun, Luying Xia, Yongzhen Biosensing Techniques Sulfides Agrobacterium tumefaciens Geologic Sediments Bacterial Proteins Environmental Monitoring Supersulfides are a class of sulfur compounds in either zero-valent or monovalent negative states, such as elemental sulfur, inorganic polysulfides, and organic polysulfides, and they play crucial roles in biological systems, environmental sulfur cycling, and Li-S batteries. However, accurate quantification of these compounds remains challenging due to their reactive nature and structural complexity. Here, we report the first whole-cell biosensor for the specific detection of supersulfides using the supersulfide-sensing repressor BigR of Agrobacterium tumefaciens (AtBigR). This novel biosensor system shows exceptional selectivity for supersulfides with negligible response to HS, thiosulfate, and glutathione. Through systematic optimization of the AtBigR-based whole-cell biosensor (AtBigR-WCB), we developed a biosensor with negligible background leakage and high sensitivity, capable of detecting supersulfides at micromolar levels. We successfully applied AtBigR-WCB to quantify supersulfide contents in marine sediments from diverse seafloor topographies and lithium polysulfides in lithium-sulfur battery electrolytes. Widespread supersulfide accumulation was detected in deeper layers of marine sediments across various environments. Additionally, the optimized AtBigR regulatory system was a novel, low-leakage regulating system, which offers a new type of gene-regulating system with potential applications in synthetic biology. Our work establishes a new platform for quantifying supersulfides, which is helpful to further our understanding of supersulfides in biology, environments, and lithium sulfur batteries. |
| title | Development and application of a highly specific whole-cell biosensor for supersulfide detection in environmental samples. |
| topic | Biosensing Techniques Sulfides Agrobacterium tumefaciens Geologic Sediments Bacterial Proteins Environmental Monitoring |
| url | https://pubmed.ncbi.nlm.nih.gov/40592263/ |