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| Format: | Artículo científico |
| Sprache: | en |
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Communications biology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40593336/ |
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| author | Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A |
| author_facet | Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A |
| collection | PubMed - marine biology |
| contents | Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish. Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A Animals Zebrafish Rhombencephalon Zebrafish Proteins Monocarboxylic Acid Transporters Thyroid Hormones Gene Expression Regulation, Developmental Symporters Female Vascular Endothelial Growth Factor A Thyroid hormone (TH) signaling is important and necessary for proper neurodevelopment. Inadequate levels of maternally derived THs (MTH) supply affect target gene expression profiles, which are fundamental for the brain's normal growth, maturation, and function. The monocarboxylate transporter 8 (SLC16A2, MCT8) is the main TH transporter present in the brain during embryonic development, and mutations in this transporter lead to a rare and debilitating human condition known as the Allan-Herndon-Dudley Syndrome (AHDS). This mutation affects the capacity for intracellular transport of the hormone, leading to impaired brain development that constitutes the main pathophysiological basis of AHDS. Like humans, zebrafish embryos express slc16a2 that transports exclusively T3 at zebrafish physiological temperature. Studies in zebrafish Mct8 knockdown (KD) models found impaired hindbrain vasculature development. Here, using zebrafish Mct8 KD and knockout (KO) models, we shed light on the maternal T3 (MT3)-dependent developmental mechanism behind hindbrain vasculature development. We first demonstrate that MT3-regulates hindbrain vegfaa expression. We provide evidence that hindbrain neurons are not the source of vegfaa, instead, restricted pax6a+ neuroprogenitor cells (NPCs) instruct central arteries (CtAs) ingression into the hindbrain. Therefore, MT3 acts as an integrator, providing the regulatory cues necessary for the timely ingression of the CtAs into the hindbrain. |
| format | Artículo científico |
| id | pubmed_40593336 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Communications biology |
| record_format | pubmed |
| spellingShingle | Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish. Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A Animals Zebrafish Rhombencephalon Zebrafish Proteins Monocarboxylic Acid Transporters Thyroid Hormones Gene Expression Regulation, Developmental Symporters Female Vascular Endothelial Growth Factor A Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish. Trindade, Marlene Silva, Nádia Rodrigues, Joana Kawakami, Koichi Campinho, Marco A Animals Zebrafish Rhombencephalon Zebrafish Proteins Monocarboxylic Acid Transporters Thyroid Hormones Gene Expression Regulation, Developmental Symporters Female Vascular Endothelial Growth Factor A Thyroid hormone (TH) signaling is important and necessary for proper neurodevelopment. Inadequate levels of maternally derived THs (MTH) supply affect target gene expression profiles, which are fundamental for the brain's normal growth, maturation, and function. The monocarboxylate transporter 8 (SLC16A2, MCT8) is the main TH transporter present in the brain during embryonic development, and mutations in this transporter lead to a rare and debilitating human condition known as the Allan-Herndon-Dudley Syndrome (AHDS). This mutation affects the capacity for intracellular transport of the hormone, leading to impaired brain development that constitutes the main pathophysiological basis of AHDS. Like humans, zebrafish embryos express slc16a2 that transports exclusively T3 at zebrafish physiological temperature. Studies in zebrafish Mct8 knockdown (KD) models found impaired hindbrain vasculature development. Here, using zebrafish Mct8 KD and knockout (KO) models, we shed light on the maternal T3 (MT3)-dependent developmental mechanism behind hindbrain vasculature development. We first demonstrate that MT3-regulates hindbrain vegfaa expression. We provide evidence that hindbrain neurons are not the source of vegfaa, instead, restricted pax6a+ neuroprogenitor cells (NPCs) instruct central arteries (CtAs) ingression into the hindbrain. Therefore, MT3 acts as an integrator, providing the regulatory cues necessary for the timely ingression of the CtAs into the hindbrain. |
| title | Maternal thyroid hormone is required to develop the hindbrain vasculature in zebrafish. |
| topic | Animals Zebrafish Rhombencephalon Zebrafish Proteins Monocarboxylic Acid Transporters Thyroid Hormones Gene Expression Regulation, Developmental Symporters Female Vascular Endothelial Growth Factor A |
| url | https://pubmed.ncbi.nlm.nih.gov/40593336/ |