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| Sprache: | en |
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Scientific data
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40593763/ |
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| author | McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed |
| author_facet | McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed |
| collection | PubMed - marine biology |
| contents | Characterizing organisms from three domains of life with universal primers from throughout the global ocean. McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed Archaea Bacteria Eukaryota Seawater Oceans and Seas DNA Barcoding, Taxonomic DNA Primers Plankton We introduce the Global rRNA Universal Metabarcoding Plankton database (GRUMP), which consists of 1194 samples that were collected from 2003-2020 and cover extensive latitudinal and longitudinal transects, as well as depth profiles in all major ocean basins. DNA from unfractionated (>0.2 µm) seawater samples was amplified using the 515Y/926 R universal three-domain rRNA gene primers, simultaneously quantifying the relative abundance of amplicon sequencing variants (ASVs) from bacteria, archaea, eukaryotic nuclear 18S, and eukaryotic plastid 16S. Thus, the ratio between taxa in one sample is directly comparable to the ratio in any other GRUMP sample, regardless of gene copy number differences. This obviates a problem in prior global studies that used size-fractionation and different rRNA gene primers for bacteria, archaea, and eukaryotes, precluding comparisons across size fractions or domains. On average, bacteria contributed 71%, eukaryotes 19%, and archaea 8% to rRNA gene abundance, though eukaryotes contributed 32% at latitudes >40°. GRUMP is publicly available on the Simons Collaborative Marine Atlas Project (CMAP), promoting the global comparison of marine microbial dynamics. |
| format | Artículo científico |
| id | pubmed_40593763 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Scientific data |
| record_format | pubmed |
| spellingShingle | Characterizing organisms from three domains of life with universal primers from throughout the global ocean. McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed Archaea Bacteria Eukaryota Seawater Oceans and Seas DNA Barcoding, Taxonomic DNA Primers Plankton Characterizing organisms from three domains of life with universal primers from throughout the global ocean. McNichol, Jesse Williams, Nathan L R Raut, Yubin Carlson, Craig Halewood, Elisa R Turk-Kubo, Kendra Zehr, Jonathan P Rees, Andrew P Tarran, Glen Gradoville, Mary R Wietz, Matthias Bienhold, Christina Metfies, Katja Torres-Valdés, Sinhué Mock, Thomas Eggers, Sarah Lena Jeffrey, Wade Moss, Joseph Berube, Paul Biller, Steven Bodrossy, Levente Van De Kamp, Jodie Brown, Mark Sow, Swan L S Armbrust, E Virginia Fuhrman, Jed Archaea Bacteria Eukaryota Seawater Oceans and Seas DNA Barcoding, Taxonomic DNA Primers Plankton We introduce the Global rRNA Universal Metabarcoding Plankton database (GRUMP), which consists of 1194 samples that were collected from 2003-2020 and cover extensive latitudinal and longitudinal transects, as well as depth profiles in all major ocean basins. DNA from unfractionated (>0.2 µm) seawater samples was amplified using the 515Y/926 R universal three-domain rRNA gene primers, simultaneously quantifying the relative abundance of amplicon sequencing variants (ASVs) from bacteria, archaea, eukaryotic nuclear 18S, and eukaryotic plastid 16S. Thus, the ratio between taxa in one sample is directly comparable to the ratio in any other GRUMP sample, regardless of gene copy number differences. This obviates a problem in prior global studies that used size-fractionation and different rRNA gene primers for bacteria, archaea, and eukaryotes, precluding comparisons across size fractions or domains. On average, bacteria contributed 71%, eukaryotes 19%, and archaea 8% to rRNA gene abundance, though eukaryotes contributed 32% at latitudes >40°. GRUMP is publicly available on the Simons Collaborative Marine Atlas Project (CMAP), promoting the global comparison of marine microbial dynamics. |
| title | Characterizing organisms from three domains of life with universal primers from throughout the global ocean. |
| topic | Archaea Bacteria Eukaryota Seawater Oceans and Seas DNA Barcoding, Taxonomic DNA Primers Plankton |
| url | https://pubmed.ncbi.nlm.nih.gov/40593763/ |