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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Scientific data
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40593801/ |
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Table of Contents:
- De novo transcriptome assembly of Acartia tonsa adults using Nanopore long-read sequencing. Mohamed, Florencia Picone, Marco Battaggia, Greta Urso, Ilenia Camatti, Elisa Vezzi, Alessandro Sales, Gabriele Transcriptome Animals Copepoda Nanopore Sequencing Neonicotinoids Molecular Sequence Annotation Acartia tonsa is a calanoid copepod with a cosmopolitan distribution. Its ecological relevance makes it a useful bioindicator organism for assessing the toxicity of various compounds. However, transcriptomic assemblies of A. tonsa using long-read technologies have not yet been described. The use of long-read sequencing technologies in transcriptomics allows the study of alternative splicing, structural variations and alternative polyadenylation sites. In this study, we present a de novo transcriptome of A. tonsa adult copepods exposed to different neonicotinoids obtained from Nanopore sequences. This transcriptome (261,560 total transcripts, with 31,291 representative sequences) exhibits 88.3% completeness and an N50 of 2,580 bases, showing better results than previous assemblies of the same organism. We also performed a full annotation by sequence homology (NR database), domain identification (InterProScan) and functional classification (Gene Ontology); 54.3% of representative transcripts were annotated in at least one database. Our transcriptome represents a solid baseline for further transcriptomic studies on A. tonsa and, specifically, its response to currently used pesticides.