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Hauptverfasser: Ahammad, A K Shakur, Abid, Md Baktiar, Ahmed, Ilias, Mariom, Khan, Mohd Golam Quader, Haque, Mohammad Mahfujul, Wong, Li Lian, Alam, Mohammad Ashraful, Bashar, Md Abul
Format: Artículo científico
Sprache:en
Veröffentlicht: Scientific reports 2025
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Online-Zugang:https://pubmed.ncbi.nlm.nih.gov/40594873/
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author Ahammad, A K Shakur
Abid, Md Baktiar
Ahmed, Ilias
Mariom
Khan, Mohd Golam Quader
Haque, Mohammad Mahfujul
Wong, Li Lian
Alam, Mohammad Ashraful
Bashar, Md Abul
author_facet Ahammad, A K Shakur
Abid, Md Baktiar
Ahmed, Ilias
Mariom
Khan, Mohd Golam Quader
Haque, Mohammad Mahfujul
Wong, Li Lian
Alam, Mohammad Ashraful
Bashar, Md Abul
Ahammad, A K Shakur
Abid, Md Baktiar
Ahmed, Ilias
Mariom
Khan, Mohd Golam Quader
Haque, Mohammad Mahfujul
Wong, Li Lian
Alam, Mohammad Ashraful
Bashar, Md Abul
collection PubMed - marine biology
contents Transcriptomic profiling to unravel the underlying causes of early gonadal development of hilsa (Tenualosa ilisha) in Bangladesh. Ahammad, A K Shakur Abid, Md Baktiar Ahmed, Ilias Mariom Khan, Mohd Golam Quader Haque, Mohammad Mahfujul Wong, Li Lian Alam, Mohammad Ashraful Bashar, Md Abul Female Gene Expression Profiling Bangladesh Transcriptome Animals Ovary Gene Expression Regulation, Developmental Gene Ontology Hilsa (Tenualosa ilisha) is crucial to Bangladesh's economy and ecology, but recent trends show early maturation in small-sized hilsa, with unclear causes. This study aimed to identify differentially expressed genes (DEGs) between the ovaries of large and small hilsa using RNA-sequencing. Samples from both sizes were collected from the Meghna River in Chandpur District, and four ovary libraries were sequenced via the Illumina platform. A total of 908 DEGs were detected, of which 453 were up-regulated and 455 were down-regulated. Transcriptome analysis revealed significant differences in Gene Ontology terms such as extracellular region (GO:0005576), innate immune response (GO:0045087), and protein ubiquitination (GO:0016567), peptide antigen binding (GO:0042605), KEGG pathways including MAPK signaling pathway (ko04010), endocytosis (ko04144), and spliceosome (ko03040), as well as COG terms like signal transduction, between the ovary of large and small hilsa shad. These findings suggest that differentially expressed genes may play a role in regulating the ovarian development of T. ilisha. However, the entire process, from sampling to final annotation, is described here, along with the workflow. This study will offer a valuable resource for current and upcoming Hilsa transcriptome-based expression profiling studies.
format Artículo científico
id pubmed_40594873
institution PubMed
language en
publishDate 2025
publisher Scientific reports
record_format pubmed
spellingShingle Transcriptomic profiling to unravel the underlying causes of early gonadal development of hilsa (Tenualosa ilisha) in Bangladesh.
Ahammad, A K Shakur
Abid, Md Baktiar
Ahmed, Ilias
Mariom
Khan, Mohd Golam Quader
Haque, Mohammad Mahfujul
Wong, Li Lian
Alam, Mohammad Ashraful
Bashar, Md Abul
Female
Gene Expression Profiling
Bangladesh
Transcriptome
Animals
Ovary
Gene Expression Regulation, Developmental
Gene Ontology
Transcriptomic profiling to unravel the underlying causes of early gonadal development of hilsa (Tenualosa ilisha) in Bangladesh. Ahammad, A K Shakur Abid, Md Baktiar Ahmed, Ilias Mariom Khan, Mohd Golam Quader Haque, Mohammad Mahfujul Wong, Li Lian Alam, Mohammad Ashraful Bashar, Md Abul Female Gene Expression Profiling Bangladesh Transcriptome Animals Ovary Gene Expression Regulation, Developmental Gene Ontology Hilsa (Tenualosa ilisha) is crucial to Bangladesh's economy and ecology, but recent trends show early maturation in small-sized hilsa, with unclear causes. This study aimed to identify differentially expressed genes (DEGs) between the ovaries of large and small hilsa using RNA-sequencing. Samples from both sizes were collected from the Meghna River in Chandpur District, and four ovary libraries were sequenced via the Illumina platform. A total of 908 DEGs were detected, of which 453 were up-regulated and 455 were down-regulated. Transcriptome analysis revealed significant differences in Gene Ontology terms such as extracellular region (GO:0005576), innate immune response (GO:0045087), and protein ubiquitination (GO:0016567), peptide antigen binding (GO:0042605), KEGG pathways including MAPK signaling pathway (ko04010), endocytosis (ko04144), and spliceosome (ko03040), as well as COG terms like signal transduction, between the ovary of large and small hilsa shad. These findings suggest that differentially expressed genes may play a role in regulating the ovarian development of T. ilisha. However, the entire process, from sampling to final annotation, is described here, along with the workflow. This study will offer a valuable resource for current and upcoming Hilsa transcriptome-based expression profiling studies.
title Transcriptomic profiling to unravel the underlying causes of early gonadal development of hilsa (Tenualosa ilisha) in Bangladesh.
topic Female
Gene Expression Profiling
Bangladesh
Transcriptome
Animals
Ovary
Gene Expression Regulation, Developmental
Gene Ontology
url https://pubmed.ncbi.nlm.nih.gov/40594873/