Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
BMC microbiology
2025
|
| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40604432/ |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1868266181691965440 |
|---|---|
| author | Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong |
| author_facet | Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong |
| collection | PubMed - marine biology |
| contents | Particle associated denitrification in coastal aquaculture water. Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong Denitrification Aquaculture Bacteria China Nitrates Particle Size Particulate Matter Seawater Nitrate Reductase Phylogeny Water Microbiology High-Throughput Nucleotide Sequencing The nitrate reductase S (nirS) type denitrifying bacteria is important for nitrate removal, and suspended particulate matter (SPM) in coastal aquaculture water provides a microenvironment for denitrification to occur. However, it is currently unclear about the denitrification mechanism for nitrate removal occurred on SPM in coastal aquaculture water. SPM with different particle sizes (0.22, 1 and 5 μm) were collected from coastal cage aquaculture ponds water in Maoming City, China. Gene abundance, community structure, diversity and environmental influences of nirS-type denitrifying bacteria on SPM were investigated by fluorescence quantitative PCR (qPCR), high-throughput sequencing and statistical analysis. Roseobacter was widely distributed on SPM with three kinds of particle sizes, especially SPM with 0.22 μm particle size. Pelomonas and Pseudomonas were mainly present on SPM with 1 and 0.22 μm particle sizes, while Acidithiobacillales was mainly present on SPM with 5 μm particle size. The nirS gene abundance and community diversity of denitrifying bacteria was higher on SPM with 5 μm particle size than that with 1 and 0.22 μm particle sizes. The nirS-type denitrifying bacteria communities clustered evidently according to SPM particle sizes in cage aquaculture ponds water. NO-N, NO-N, particle size, and pH were identified as significant factors affecting the community structure and diversity of nirS-type denitrifying keystone genera on SPM. The present study provided new insights into denitrification in the microenvironment on SPM in aquaculture systems, which may contribute to the biological management of cage aquaculture ponds. |
| format | Artículo científico |
| id | pubmed_40604432 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | BMC microbiology |
| record_format | pubmed |
| spellingShingle | Particle associated denitrification in coastal aquaculture water. Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong Denitrification Aquaculture Bacteria China Nitrates Particle Size Particulate Matter Seawater Nitrate Reductase Phylogeny Water Microbiology High-Throughput Nucleotide Sequencing Particle associated denitrification in coastal aquaculture water. Kuang, Chunyi Hong, Yiguo Sun, Wei Wu, Jiapeng Chen, Ruixi Wei, Mingken Chen, Lizhao Zhu, Zhidong Denitrification Aquaculture Bacteria China Nitrates Particle Size Particulate Matter Seawater Nitrate Reductase Phylogeny Water Microbiology High-Throughput Nucleotide Sequencing The nitrate reductase S (nirS) type denitrifying bacteria is important for nitrate removal, and suspended particulate matter (SPM) in coastal aquaculture water provides a microenvironment for denitrification to occur. However, it is currently unclear about the denitrification mechanism for nitrate removal occurred on SPM in coastal aquaculture water. SPM with different particle sizes (0.22, 1 and 5 μm) were collected from coastal cage aquaculture ponds water in Maoming City, China. Gene abundance, community structure, diversity and environmental influences of nirS-type denitrifying bacteria on SPM were investigated by fluorescence quantitative PCR (qPCR), high-throughput sequencing and statistical analysis. Roseobacter was widely distributed on SPM with three kinds of particle sizes, especially SPM with 0.22 μm particle size. Pelomonas and Pseudomonas were mainly present on SPM with 1 and 0.22 μm particle sizes, while Acidithiobacillales was mainly present on SPM with 5 μm particle size. The nirS gene abundance and community diversity of denitrifying bacteria was higher on SPM with 5 μm particle size than that with 1 and 0.22 μm particle sizes. The nirS-type denitrifying bacteria communities clustered evidently according to SPM particle sizes in cage aquaculture ponds water. NO-N, NO-N, particle size, and pH were identified as significant factors affecting the community structure and diversity of nirS-type denitrifying keystone genera on SPM. The present study provided new insights into denitrification in the microenvironment on SPM in aquaculture systems, which may contribute to the biological management of cage aquaculture ponds. |
| title | Particle associated denitrification in coastal aquaculture water. |
| topic | Denitrification Aquaculture Bacteria China Nitrates Particle Size Particulate Matter Seawater Nitrate Reductase Phylogeny Water Microbiology High-Throughput Nucleotide Sequencing |
| url | https://pubmed.ncbi.nlm.nih.gov/40604432/ |