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| Sprache: | en |
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Fish & shellfish immunology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40609774/ |
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| author | Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei |
| author_facet | Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei |
| collection | PubMed - marine biology |
| contents | IL-4/13A1 promotes the proliferation of IgT B cells while enhancing their apoptosis in tilapia. Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei Animals Apoptosis Cell Proliferation Fish Proteins Fish Diseases B-Lymphocytes Cichlids Interleukin-4 Gene Expression Regulation Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Interleukin-13 Interleukin-4/13 (IL-4/13) is a kind of bony fish cytokine, which is the homolog of Th2 cytokines IL-4 or IL-13 in mammals. Produced by activated T cells, these Th2 cytokines regulate B cell proliferation, survival, and antibody responses. Although IL-4/13 has been shown to participate in immune responses in bony fish, whether and how it regulates the teleost-specific IgT B cell subset, a lineage unique to bony fish remains unclear. In present study, we identified two conserved IL-4/13 from the Nile tilapia Oreochromis niloticus (OnIL-4/13A1 and OnIL-4/13A2), and suggested their wide expression in immunological tissues. OnIL-4/13A1 transcription was markedly upregulated in spleen and liver leukocytes upon bacterial infection, and can be dramatically induced by PHA- or CD3 mAb-induced T cell activation, suggesting activated T cells are a key cellular source of this cytokine. More importantly, we found that 2- or 4-days' in vivo administration of the recombinant OnIL-4/13A1 (rOnIL-4/13A1) caused an obvious expansion of IgT but not IgM B cells in tilapia. Further investigation demonstrated that this expansion was due to cellular proliferation, because the BrdU incorporation in IgT B cells was obviously increased, while the Erk1/2 and NF-κB phosphorylation that contributing to the proliferation was also enhanced in IgT B cells. Interestingly, as revealed by the Annexin V staining, we found that rOnIL-4/13A1 treatment elevated the apoptosis of IgT B cells, probably by initiating the Caspase-8/Caspase-3 pathway. Therefore, our results suggest that IL-4/13A1 involves in the anti-bacterial immune response of tilapia by promoting IgT B cell proliferation and maintaining IgT B cell hemostasis, thus shed novel light on the B cells response in bony fish. |
| format | Artículo científico |
| id | pubmed_40609774 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | IL-4/13A1 promotes the proliferation of IgT B cells while enhancing their apoptosis in tilapia. Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei Animals Apoptosis Cell Proliferation Fish Proteins Fish Diseases B-Lymphocytes Cichlids Interleukin-4 Gene Expression Regulation Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Interleukin-13 IL-4/13A1 promotes the proliferation of IgT B cells while enhancing their apoptosis in tilapia. Zhu, Yating Chen, Haokai Zheng, Yuying Zhang, Jiansong Geng, Ming Fang, Zhichao Li, Kang Yang, Jialong Wei, Xiumei Animals Apoptosis Cell Proliferation Fish Proteins Fish Diseases B-Lymphocytes Cichlids Interleukin-4 Gene Expression Regulation Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Interleukin-13 Interleukin-4/13 (IL-4/13) is a kind of bony fish cytokine, which is the homolog of Th2 cytokines IL-4 or IL-13 in mammals. Produced by activated T cells, these Th2 cytokines regulate B cell proliferation, survival, and antibody responses. Although IL-4/13 has been shown to participate in immune responses in bony fish, whether and how it regulates the teleost-specific IgT B cell subset, a lineage unique to bony fish remains unclear. In present study, we identified two conserved IL-4/13 from the Nile tilapia Oreochromis niloticus (OnIL-4/13A1 and OnIL-4/13A2), and suggested their wide expression in immunological tissues. OnIL-4/13A1 transcription was markedly upregulated in spleen and liver leukocytes upon bacterial infection, and can be dramatically induced by PHA- or CD3 mAb-induced T cell activation, suggesting activated T cells are a key cellular source of this cytokine. More importantly, we found that 2- or 4-days' in vivo administration of the recombinant OnIL-4/13A1 (rOnIL-4/13A1) caused an obvious expansion of IgT but not IgM B cells in tilapia. Further investigation demonstrated that this expansion was due to cellular proliferation, because the BrdU incorporation in IgT B cells was obviously increased, while the Erk1/2 and NF-κB phosphorylation that contributing to the proliferation was also enhanced in IgT B cells. Interestingly, as revealed by the Annexin V staining, we found that rOnIL-4/13A1 treatment elevated the apoptosis of IgT B cells, probably by initiating the Caspase-8/Caspase-3 pathway. Therefore, our results suggest that IL-4/13A1 involves in the anti-bacterial immune response of tilapia by promoting IgT B cell proliferation and maintaining IgT B cell hemostasis, thus shed novel light on the B cells response in bony fish. |
| title | IL-4/13A1 promotes the proliferation of IgT B cells while enhancing their apoptosis in tilapia. |
| topic | Animals Apoptosis Cell Proliferation Fish Proteins Fish Diseases B-Lymphocytes Cichlids Interleukin-4 Gene Expression Regulation Phylogeny Sequence Alignment Amino Acid Sequence Immunity, Innate Interleukin-13 |
| url | https://pubmed.ncbi.nlm.nih.gov/40609774/ |