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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
International journal of systematic and evolutionary microbiology
2025
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| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40622755/ |
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| author | Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan |
| author_facet | Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan |
| collection | PubMed - marine biology |
| contents | sp. nov., a novel species isolated on a cruise ship during routine monitoring. Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan RNA, Ribosomal, 16S Legionella Fatty Acids Phylogeny Bacterial Typing Techniques DNA, Bacterial Anti-Bacterial Agents Microbial Sensitivity Tests Ships Base Composition Whole Genome Sequencing Seawater Nucleic Acid Hybridization Genome, Bacterial Sequence Analysis, DNA like isolates, HB10 and PATHC038, were isolated from a cold water distribution system on a cruise ship in 2017. The strains have been characterized by employing discriminatory genome typing, matrix-assisted laser desorption/ionization time-of-flight MS technique, fatty acid profiling and biochemical tests, thus identifying a new species in the genus . Colonies displayed phenotypic characteristics typical of the family , including a requirement for l-cysteine and testing catalase positive. Fatty acid methyl ester analysis showed that the predominant fatty acids detected in the studied strains are C16:0 iso, C16:1 7, C16:0 and C15:0 anteiso. The obtained MIC values showed antimicrobial susceptibility to the antimicrobial drugs ciprofloxacin and erythromycin (0.8 and 2 µg ml, respectively). The bacteria were also Gram-negative, rod-shaped, grew aerobically on buffered charcoal yeast extract agar and weakly grew on glycine-vancomycin-polymyxin-cycloheximide media at 36 °C with the ability to autofluoresce a blue-white colour when placed under a long-wavelength UV light (365 nm). The whole-genome sequencing performed displayed a G+C content of 38.2 mol%. The digital DNA-DNA hybridization analysis demonstrated a separation from the phylogenetically most related , with 54.7% DNA-DNA relatedness. The identity percentage measured by average nucleotide identity between the PATHC038 strain and its respective closest species, , was 93.9%, also confirming the distinctiveness of the novel species. The 16S rRNA gene, and sequences showed a similarity of 98.7, 98.4 and 96.2%, respectively, with NCTC 11976. Additionally, average amino acid identity and percentage of conserved proteins analyses further supported their classification as a novel species. The results obtained in this study confirm the status of an independent species. The name proposed for this species is sp. nov. with PATHC038 (CCUG 76918T, ATCC TSD-370) as the type strain. |
| format | Artículo científico |
| id | pubmed_40622755 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | sp. nov., a novel species isolated on a cruise ship during routine monitoring. Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan RNA, Ribosomal, 16S Legionella Fatty Acids Phylogeny Bacterial Typing Techniques DNA, Bacterial Anti-Bacterial Agents Microbial Sensitivity Tests Ships Base Composition Whole Genome Sequencing Seawater Nucleic Acid Hybridization Genome, Bacterial Sequence Analysis, DNA sp. nov., a novel species isolated on a cruise ship during routine monitoring. Sauerborn Klobucar, Roberta Kovačević, Maja Svetlicic, Ema Kasalo, Niko Vasari, Karla Santic, Marina Antonic, Masa Posta, Ana Ivančić Šantek, Mirela Stopić, Katarina Kazazic, Snjezana Malev, Olga Kapetanovic, Damir Vucić, Matej Begic, Karolina Franjevic, Damjan Klobučar, Göran Mijakovic, Ivan RNA, Ribosomal, 16S Legionella Fatty Acids Phylogeny Bacterial Typing Techniques DNA, Bacterial Anti-Bacterial Agents Microbial Sensitivity Tests Ships Base Composition Whole Genome Sequencing Seawater Nucleic Acid Hybridization Genome, Bacterial Sequence Analysis, DNA like isolates, HB10 and PATHC038, were isolated from a cold water distribution system on a cruise ship in 2017. The strains have been characterized by employing discriminatory genome typing, matrix-assisted laser desorption/ionization time-of-flight MS technique, fatty acid profiling and biochemical tests, thus identifying a new species in the genus . Colonies displayed phenotypic characteristics typical of the family , including a requirement for l-cysteine and testing catalase positive. Fatty acid methyl ester analysis showed that the predominant fatty acids detected in the studied strains are C16:0 iso, C16:1 7, C16:0 and C15:0 anteiso. The obtained MIC values showed antimicrobial susceptibility to the antimicrobial drugs ciprofloxacin and erythromycin (0.8 and 2 µg ml, respectively). The bacteria were also Gram-negative, rod-shaped, grew aerobically on buffered charcoal yeast extract agar and weakly grew on glycine-vancomycin-polymyxin-cycloheximide media at 36 °C with the ability to autofluoresce a blue-white colour when placed under a long-wavelength UV light (365 nm). The whole-genome sequencing performed displayed a G+C content of 38.2 mol%. The digital DNA-DNA hybridization analysis demonstrated a separation from the phylogenetically most related , with 54.7% DNA-DNA relatedness. The identity percentage measured by average nucleotide identity between the PATHC038 strain and its respective closest species, , was 93.9%, also confirming the distinctiveness of the novel species. The 16S rRNA gene, and sequences showed a similarity of 98.7, 98.4 and 96.2%, respectively, with NCTC 11976. Additionally, average amino acid identity and percentage of conserved proteins analyses further supported their classification as a novel species. The results obtained in this study confirm the status of an independent species. The name proposed for this species is sp. nov. with PATHC038 (CCUG 76918T, ATCC TSD-370) as the type strain. |
| title | sp. nov., a novel species isolated on a cruise ship during routine monitoring. |
| topic | RNA, Ribosomal, 16S Legionella Fatty Acids Phylogeny Bacterial Typing Techniques DNA, Bacterial Anti-Bacterial Agents Microbial Sensitivity Tests Ships Base Composition Whole Genome Sequencing Seawater Nucleic Acid Hybridization Genome, Bacterial Sequence Analysis, DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/40622755/ |