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author Gomes, Luana S
Cirne-Santos, Claudio C
de S Barros, Caroline
Batista, Rafael R
de P Ignacio, Matheus R
de Oliveira, Aldo S
Ronconi, Célia M
de Palmer Paixão, Izabel C N
Nascimento, Vanessa
author_facet Gomes, Luana S
Cirne-Santos, Claudio C
de S Barros, Caroline
Batista, Rafael R
de P Ignacio, Matheus R
de Oliveira, Aldo S
Ronconi, Célia M
de Palmer Paixão, Izabel C N
Nascimento, Vanessa
Gomes, Luana S
Cirne-Santos, Claudio C
de S Barros, Caroline
Batista, Rafael R
de P Ignacio, Matheus R
de Oliveira, Aldo S
Ronconi, Célia M
de Palmer Paixão, Izabel C N
Nascimento, Vanessa
collection PubMed - marine biology
contents Sulfur/selenium-functionalized benzotriazoles as multifunctional antivirals targeting Zika & Chikungunya. Gomes, Luana S Cirne-Santos, Claudio C de S Barros, Caroline Batista, Rafael R de P Ignacio, Matheus R de Oliveira, Aldo S Ronconi, Célia M de Palmer Paixão, Izabel C N Nascimento, Vanessa Antiviral Agents Chikungunya virus Zika Virus Animals Chlorocebus aethiops Vero Cells Triazoles Molecular Docking Simulation Virus Replication Selenium Sulfur Chikungunya Fever Structure-Activity Relationship Density Functional Theory Microbial Sensitivity Tests Humans Zika Virus Infection Emerging arboviruses such as Zika virus (ZIKV) and Chikungunya virus (CHIKV) remain significant public health threats. This study aimed to evaluate the antiviral potential of six organochalcogen compounds against ZIKV and CHIKV. Compounds were assessed for cytotoxicity and antiviral activity in Vero cells. Antiviral effects were determined using plaque reduction assays, time-of-addition studies, viral adsorption, and virucidal assays. Molecular docking and density functional theory (DFT) calculations were performed to investigate interactions with viral targets and electronic properties. Compounds , , , and exhibited potent antiviral activity with low cytotoxicity, demonstrating effective inhibition of viral replication with half-maximal effective concentration (EC₅₀) values in the micromolar range and favorable selectivity indices. Mechanistic assays revealed that the compounds interfered with viral adsorption, exhibited virucidal effects, and inhibited multiple stages of the replication cycle. Docking studies confirmed strong binding to key viral enzymes, supported by HOMO (half-maximal effective concentration) - LUMO (lowest unoccupied molecular orbital) analysis. These findings highlight organochalcogen compounds as promising dual-action antiviral candidates with broad-spectrum activity against ZIKV and CHIKV. Further preclinical investigations are warranted to explore their therapeutic potential.
format Artículo científico
id pubmed_40624932
institution PubMed
language en
publishDate 2025
publisher Future medicinal chemistry
record_format pubmed
spellingShingle Sulfur/selenium-functionalized benzotriazoles as multifunctional antivirals targeting Zika & Chikungunya.
Gomes, Luana S
Cirne-Santos, Claudio C
de S Barros, Caroline
Batista, Rafael R
de P Ignacio, Matheus R
de Oliveira, Aldo S
Ronconi, Célia M
de Palmer Paixão, Izabel C N
Nascimento, Vanessa
Antiviral Agents
Chikungunya virus
Zika Virus
Animals
Chlorocebus aethiops
Vero Cells
Triazoles
Molecular Docking Simulation
Virus Replication
Selenium
Sulfur
Chikungunya Fever
Structure-Activity Relationship
Density Functional Theory
Microbial Sensitivity Tests
Humans
Zika Virus Infection
Sulfur/selenium-functionalized benzotriazoles as multifunctional antivirals targeting Zika & Chikungunya. Gomes, Luana S Cirne-Santos, Claudio C de S Barros, Caroline Batista, Rafael R de P Ignacio, Matheus R de Oliveira, Aldo S Ronconi, Célia M de Palmer Paixão, Izabel C N Nascimento, Vanessa Antiviral Agents Chikungunya virus Zika Virus Animals Chlorocebus aethiops Vero Cells Triazoles Molecular Docking Simulation Virus Replication Selenium Sulfur Chikungunya Fever Structure-Activity Relationship Density Functional Theory Microbial Sensitivity Tests Humans Zika Virus Infection Emerging arboviruses such as Zika virus (ZIKV) and Chikungunya virus (CHIKV) remain significant public health threats. This study aimed to evaluate the antiviral potential of six organochalcogen compounds against ZIKV and CHIKV. Compounds were assessed for cytotoxicity and antiviral activity in Vero cells. Antiviral effects were determined using plaque reduction assays, time-of-addition studies, viral adsorption, and virucidal assays. Molecular docking and density functional theory (DFT) calculations were performed to investigate interactions with viral targets and electronic properties. Compounds , , , and exhibited potent antiviral activity with low cytotoxicity, demonstrating effective inhibition of viral replication with half-maximal effective concentration (EC₅₀) values in the micromolar range and favorable selectivity indices. Mechanistic assays revealed that the compounds interfered with viral adsorption, exhibited virucidal effects, and inhibited multiple stages of the replication cycle. Docking studies confirmed strong binding to key viral enzymes, supported by HOMO (half-maximal effective concentration) - LUMO (lowest unoccupied molecular orbital) analysis. These findings highlight organochalcogen compounds as promising dual-action antiviral candidates with broad-spectrum activity against ZIKV and CHIKV. Further preclinical investigations are warranted to explore their therapeutic potential.
title Sulfur/selenium-functionalized benzotriazoles as multifunctional antivirals targeting Zika & Chikungunya.
topic Antiviral Agents
Chikungunya virus
Zika Virus
Animals
Chlorocebus aethiops
Vero Cells
Triazoles
Molecular Docking Simulation
Virus Replication
Selenium
Sulfur
Chikungunya Fever
Structure-Activity Relationship
Density Functional Theory
Microbial Sensitivity Tests
Humans
Zika Virus Infection
url https://pubmed.ncbi.nlm.nih.gov/40624932/