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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
mSystems
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40626787/ |
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| author | Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn |
| author_facet | Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn |
| collection | PubMed - marine biology |
| contents | Structural and regulatory determinants of flagellar motility in -the archetypal flagellum of DSM 17395. Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn Flagella Bacterial Proteins Phylogeny Gene Expression Regulation, Bacterial Multigene Family Rhodobacteraceae Flagellar motility is crucial for the swim-and-stick lifestyle and plays an important role in bacterial-algal interactions of . This alphaproteobacterial order contains three distinct types of flagellar gene clusters (FGCs) for the formation of a functional flagellum. Our phylogenetically broad taxon sampling of more than 300 genomes revealed that the most common FGC, the -type, was probably already present in the common ancestor of and was strictly vertically inherited, while the other two FGC types, 2 and , were spread via horizontal operon transfers. Swimming of the marine model organism DSM 17395 () is mediated by the archetypal -type flagellum. Screening of 13,000 transposon mutants of on soft agar plates revealed that 40 genes, including four genes encoding conserved but not yet characterized proteins (CP1-4) within the FGC, are essential for motility. Exoproteome analyses indicated that CP1-4 are required at different stages of flagellar assembly. Only eight genes outside the FGC were identified as essential for swimming motility, including all three genes of the CtrA phosphorelay. Using comparative transcriptomics of Δ, Δ, and Δ mutants of the distantly related model organisms and DSM 16493, we identified genes for the flagellum and cyclic di-GMP turnover as core targets of the CtrA phosphorelay and a conserved connection with quorum sensing across members of the . The bacterial flagellum is a sophisticated nanomachine for swimming motility and rapid chemotactic response to gradients of attractants or repellents in the environment. It is structurally highly conserved and has been intensively studied in gammaproteobacterial model bacteria such as and . However, the flagellar gene clusters of different alphaproteobacterial orders have distinct structures and compositions, as demonstrated by the three flagellar systems of investigated in the current study. The archetypal -type flagellum originated in its common ancestor and evolved synchronously with the host. The universal presence of four as yet uncharacterized essential genes in -type FGCs (CP1-4) reflects the order-specific adaptation of the flagellar system during bacterial evolution. Comparative transcriptome analyses of Δ, Δ and Δ mutants showed that the core function of the CtrA phosphorelay in is the transcriptional control of flagellar genes. |
| format | Artículo científico |
| id | pubmed_40626787 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | mSystems |
| record_format | pubmed |
| spellingShingle | Structural and regulatory determinants of flagellar motility in -the archetypal flagellum of DSM 17395. Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn Flagella Bacterial Proteins Phylogeny Gene Expression Regulation, Bacterial Multigene Family Rhodobacteraceae Structural and regulatory determinants of flagellar motility in -the archetypal flagellum of DSM 17395. Tomasch, Jürgen Bartling, Pascal Vollmers, John Wöhlbrand, Lars Jarek, Michael Rohde, Manfred Brinkmann, Henner Freese, Heike M Rabus, Ralf Petersen, Jörn Flagella Bacterial Proteins Phylogeny Gene Expression Regulation, Bacterial Multigene Family Rhodobacteraceae Flagellar motility is crucial for the swim-and-stick lifestyle and plays an important role in bacterial-algal interactions of . This alphaproteobacterial order contains three distinct types of flagellar gene clusters (FGCs) for the formation of a functional flagellum. Our phylogenetically broad taxon sampling of more than 300 genomes revealed that the most common FGC, the -type, was probably already present in the common ancestor of and was strictly vertically inherited, while the other two FGC types, 2 and , were spread via horizontal operon transfers. Swimming of the marine model organism DSM 17395 () is mediated by the archetypal -type flagellum. Screening of 13,000 transposon mutants of on soft agar plates revealed that 40 genes, including four genes encoding conserved but not yet characterized proteins (CP1-4) within the FGC, are essential for motility. Exoproteome analyses indicated that CP1-4 are required at different stages of flagellar assembly. Only eight genes outside the FGC were identified as essential for swimming motility, including all three genes of the CtrA phosphorelay. Using comparative transcriptomics of Δ, Δ, and Δ mutants of the distantly related model organisms and DSM 16493, we identified genes for the flagellum and cyclic di-GMP turnover as core targets of the CtrA phosphorelay and a conserved connection with quorum sensing across members of the . The bacterial flagellum is a sophisticated nanomachine for swimming motility and rapid chemotactic response to gradients of attractants or repellents in the environment. It is structurally highly conserved and has been intensively studied in gammaproteobacterial model bacteria such as and . However, the flagellar gene clusters of different alphaproteobacterial orders have distinct structures and compositions, as demonstrated by the three flagellar systems of investigated in the current study. The archetypal -type flagellum originated in its common ancestor and evolved synchronously with the host. The universal presence of four as yet uncharacterized essential genes in -type FGCs (CP1-4) reflects the order-specific adaptation of the flagellar system during bacterial evolution. Comparative transcriptome analyses of Δ, Δ and Δ mutants showed that the core function of the CtrA phosphorelay in is the transcriptional control of flagellar genes. |
| title | Structural and regulatory determinants of flagellar motility in -the archetypal flagellum of DSM 17395. |
| topic | Flagella Bacterial Proteins Phylogeny Gene Expression Regulation, Bacterial Multigene Family Rhodobacteraceae |
| url | https://pubmed.ncbi.nlm.nih.gov/40626787/ |