Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine environmental research
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40627889/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266181652119552 |
|---|---|
| author | Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A |
| author_facet | Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A |
| collection | PubMed - marine biology |
| contents | Energy metabolism under thermal stress in Mytilus galloprovincialis and Ruditapes decussatus: Insights from gene expression and enzyme activity profiles. Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A Animals Energy Metabolism Mytilus Bivalvia Hot Temperature Stress, Physiological Gene Expression Global Warming Global warming and the associated marine heatwaves expose bivalve mollusks to conditions beyond their tolerance limits, leading to significant mortalities in fisheries and aquaculture. Bivalves have evolved efficient, though time-limited, adaptive strategies to extend survival under temperature extremes and correlated hypoxic conditions. This study investigates adjustments in key metabolic pathways regulating energy production, utilization, and balance in Mytilus galloprovincialis and Ruditapes decussatus during a 25-day exposure to increasing temperatures. The transcription of pyruvate kinase (pk) and phosphoenolpyruvate carboxykinase (pepck) genes, along with the enzymatic activities of citrate synthase (CS), 3-hydroxyacyl-CoA dehydrogenase (HOAD), and lactate dehydrogenase (LDH) were assessed at various time points. The two bivalves showed a restrained response to mild temperature increases. Mild warming induced a late upregulation of aerobic capacity and glycolysis in clams, while in mussels led to a sustained conservative response. At intermediate and high temperatures, both species increasingly relied on anaerobic pathways, likely to compensate for reduced ATP production, particularly in the later stages of exposure. Mid-exposure at intermediate temperatures led to increased glycolysis in both species possibly to meet elevated energy demands. Clams showed a stronger reliance on lipid metabolism, while mussels favored glycolysis under these conditions. At peak temperatures, mussels suppressed aerobic metabolism and increased anaerobic capacity, whereas clams enhanced lipid oxidation and increased both anaerobic and aerobic capacities. These results suggest shared metabolic adjustments that are likely conserved across bivalves and indicate interspecific compensatory mechanisms in energy balance regulation, likely stemming from diverse evolutionary adaptations. Future studies integrating advanced approaches such as transcriptomics, proteomics, and UHPLC-MS/MS could refine our understanding of these adaptive strategies, shedding light on species-specific thermal resilience in the face of climate change. |
| format | Artículo científico |
| id | pubmed_40627889 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine environmental research |
| record_format | pubmed |
| spellingShingle | Energy metabolism under thermal stress in Mytilus galloprovincialis and Ruditapes decussatus: Insights from gene expression and enzyme activity profiles. Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A Animals Energy Metabolism Mytilus Bivalvia Hot Temperature Stress, Physiological Gene Expression Global Warming Energy metabolism under thermal stress in Mytilus galloprovincialis and Ruditapes decussatus: Insights from gene expression and enzyme activity profiles. Papadopoulos, Dimitrios K Georgoulis, Ioannis Feidantsis, Konstantinos Michaelidis, Basile Giantsis, Ioannis A Animals Energy Metabolism Mytilus Bivalvia Hot Temperature Stress, Physiological Gene Expression Global Warming Global warming and the associated marine heatwaves expose bivalve mollusks to conditions beyond their tolerance limits, leading to significant mortalities in fisheries and aquaculture. Bivalves have evolved efficient, though time-limited, adaptive strategies to extend survival under temperature extremes and correlated hypoxic conditions. This study investigates adjustments in key metabolic pathways regulating energy production, utilization, and balance in Mytilus galloprovincialis and Ruditapes decussatus during a 25-day exposure to increasing temperatures. The transcription of pyruvate kinase (pk) and phosphoenolpyruvate carboxykinase (pepck) genes, along with the enzymatic activities of citrate synthase (CS), 3-hydroxyacyl-CoA dehydrogenase (HOAD), and lactate dehydrogenase (LDH) were assessed at various time points. The two bivalves showed a restrained response to mild temperature increases. Mild warming induced a late upregulation of aerobic capacity and glycolysis in clams, while in mussels led to a sustained conservative response. At intermediate and high temperatures, both species increasingly relied on anaerobic pathways, likely to compensate for reduced ATP production, particularly in the later stages of exposure. Mid-exposure at intermediate temperatures led to increased glycolysis in both species possibly to meet elevated energy demands. Clams showed a stronger reliance on lipid metabolism, while mussels favored glycolysis under these conditions. At peak temperatures, mussels suppressed aerobic metabolism and increased anaerobic capacity, whereas clams enhanced lipid oxidation and increased both anaerobic and aerobic capacities. These results suggest shared metabolic adjustments that are likely conserved across bivalves and indicate interspecific compensatory mechanisms in energy balance regulation, likely stemming from diverse evolutionary adaptations. Future studies integrating advanced approaches such as transcriptomics, proteomics, and UHPLC-MS/MS could refine our understanding of these adaptive strategies, shedding light on species-specific thermal resilience in the face of climate change. |
| title | Energy metabolism under thermal stress in Mytilus galloprovincialis and Ruditapes decussatus: Insights from gene expression and enzyme activity profiles. |
| topic | Animals Energy Metabolism Mytilus Bivalvia Hot Temperature Stress, Physiological Gene Expression Global Warming |
| url | https://pubmed.ncbi.nlm.nih.gov/40627889/ |