Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40631190/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266179252977665 |
|---|---|
| author | Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca |
| author_facet | Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca |
| collection | PubMed - marine biology |
| contents | Human reveal DNA-embedded ribonucleotides as a new type of epigenetic mark. Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca Ribonucleoside monophosphates (rNMPs) are abundant in DNA, but their distribution and function in human nuclear genomes remain unknown. Here, we mapped nearly one million rNMPs per genome across diverse human cell types, defining a nuclear "" with non-random distribution patterns. rNMPs are enriched in C/G-rich sequences, epigenetically marked regions, and telomeres. Conserved ribonucleotide-enriched zones (REZs) overlap with CpG islands and R-loops. rNMP concentration near transcription start sites (TSSs) correlates positively with gene expression. Wild-type cells display a broader gene-expression range than ribonuclease H2A (RNH2A) knockouts, in which loss of rNMP cleavage causes pronounced retention of embedded rG and strand-biased rC near TSSs, both increasing with gene expression. These findings establish DNA-embedded rNMPs as a novel epigenetic mark that modulates human gene expression. |
| format | Artículo científico |
| id | pubmed_40631190 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | Human reveal DNA-embedded ribonucleotides as a new type of epigenetic mark. Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca Human reveal DNA-embedded ribonucleotides as a new type of epigenetic mark. Kundnani, Deepali L Yang, Taehwan Channagiri, Tejasvi Xu, Penghao Lee, Yeunsoo Sun, Mo Martinez-Figueroa, Francisco Randhawa, Supreet Gogate, Ashlesha Jeon, Youngkyu Marsili, Stefania Newnam, Gary Lu, Yilin Park, Vivian S Tao, Sijia Ling, Justin A Schinazi, Raymond F Pursell, Zachary F Mohammed, Abdulmelik Opresko, Patricia L Freudenthal, Bret D Kim, Baek Yi, Soojin V Jonoska, Nataša Storici, Francesca Ribonucleoside monophosphates (rNMPs) are abundant in DNA, but their distribution and function in human nuclear genomes remain unknown. Here, we mapped nearly one million rNMPs per genome across diverse human cell types, defining a nuclear "" with non-random distribution patterns. rNMPs are enriched in C/G-rich sequences, epigenetically marked regions, and telomeres. Conserved ribonucleotide-enriched zones (REZs) overlap with CpG islands and R-loops. rNMP concentration near transcription start sites (TSSs) correlates positively with gene expression. Wild-type cells display a broader gene-expression range than ribonuclease H2A (RNH2A) knockouts, in which loss of rNMP cleavage causes pronounced retention of embedded rG and strand-biased rC near TSSs, both increasing with gene expression. These findings establish DNA-embedded rNMPs as a novel epigenetic mark that modulates human gene expression. |
| title | Human reveal DNA-embedded ribonucleotides as a new type of epigenetic mark. |
| url | https://pubmed.ncbi.nlm.nih.gov/40631190/ |