Global phylogeography and microdiversity of the marine diazotrophic photoautotrophs and UCYN-A.

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Hauptverfasser: Nguyen, Angie, Ustick, Lucas J, Larkin, Alyse A, Martiny, Adam C
Format: Artículo científico
Sprache:en
Veröffentlicht: mSphere 2025
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author Nguyen, Angie
Ustick, Lucas J
Larkin, Alyse A
Martiny, Adam C
author_facet Nguyen, Angie
Ustick, Lucas J
Larkin, Alyse A
Martiny, Adam C
Nguyen, Angie
Ustick, Lucas J
Larkin, Alyse A
Martiny, Adam C
collection PubMed - marine biology
contents Global phylogeography and microdiversity of the marine diazotrophic photoautotrophs and UCYN-A. Nguyen, Angie Ustick, Lucas J Larkin, Alyse A Martiny, Adam C Nitrogen Fixation Phylogeography Genetic Variation Trichodesmium Phylogeny Seawater Pacific Ocean Indian Ocean Nitrogenase Atlantic Ocean Biodiversity Photoautotrophic diazotrophs, specifically the genera and UCYN-A, play a pivotal role in marine nitrogen cycling through their capacity for nitrogen fixation. Despite their global distribution, the microdiversity and environmental drivers of these diazotrophs remain underexplored. This study provides a comprehensive analysis of the global diversity and distribution of and UCYN-A using the nitrogenase gene () as a genetic marker. We sequenced 954 samples from the Pacific, Atlantic, and Indian Oceans as part of the Bio-GO-SHIP project. Our results reveal significant phylogenetic and biogeographic differences between and within the two genera. exhibited greater microdiversity compared to UCYN-A, with clades showing region-specific distribution. clades were primarily influenced by temperature and nutrient availability. They were particularly frequent in regions of phosphorus stress. In contrast, UCYN-A was most frequently observed in regions experiencing iron stress. UCYN-A clades demonstrated more homogeneous distributions, with a single sequence variant within the UCYN-A1 clade dominating across varied environments. The biogeographic patterns and environmental correlations of and UCYN-A highlight the role of microdiversity in their ecological adaptation and reflect their different ecological strategies. These findings underscore the importance of characterizing the global patterns of fine-scale genetic diversity to better understand the functional roles and distribution of marine nitrogen-fixing photoautotrophs.IMPORTANCEThis study provides insights into the global diversity and distribution of nitrogen-fixing photoautotrophs, specifically and UCYN-A. We sequenced 954 oceanic samples of the nitrogenase gene and uncovered significant differences in microdiversity and environmental associations between these genera. showed high levels of sequence diversity and region-specific clades influenced by temperature and nutrient availability. In contrast, UCYN-A exhibited a more uniform distribution, thriving in iron-stressed regions. Quantifying these fine-scale genetic variations enhances our knowledge of their ecological roles and adaptations, emphasizing the need to characterize the genetic diversity of marine nitrogen-fixing prokaryotes.
format Artículo científico
id pubmed_40643242
institution PubMed
language en
publishDate 2025
publisher mSphere
record_format pubmed
spellingShingle Global phylogeography and microdiversity of the marine diazotrophic photoautotrophs and UCYN-A.
Nguyen, Angie
Ustick, Lucas J
Larkin, Alyse A
Martiny, Adam C
Nitrogen Fixation
Phylogeography
Genetic Variation
Trichodesmium
Phylogeny
Seawater
Pacific Ocean
Indian Ocean
Nitrogenase
Atlantic Ocean
Biodiversity
Global phylogeography and microdiversity of the marine diazotrophic photoautotrophs and UCYN-A. Nguyen, Angie Ustick, Lucas J Larkin, Alyse A Martiny, Adam C Nitrogen Fixation Phylogeography Genetic Variation Trichodesmium Phylogeny Seawater Pacific Ocean Indian Ocean Nitrogenase Atlantic Ocean Biodiversity Photoautotrophic diazotrophs, specifically the genera and UCYN-A, play a pivotal role in marine nitrogen cycling through their capacity for nitrogen fixation. Despite their global distribution, the microdiversity and environmental drivers of these diazotrophs remain underexplored. This study provides a comprehensive analysis of the global diversity and distribution of and UCYN-A using the nitrogenase gene () as a genetic marker. We sequenced 954 samples from the Pacific, Atlantic, and Indian Oceans as part of the Bio-GO-SHIP project. Our results reveal significant phylogenetic and biogeographic differences between and within the two genera. exhibited greater microdiversity compared to UCYN-A, with clades showing region-specific distribution. clades were primarily influenced by temperature and nutrient availability. They were particularly frequent in regions of phosphorus stress. In contrast, UCYN-A was most frequently observed in regions experiencing iron stress. UCYN-A clades demonstrated more homogeneous distributions, with a single sequence variant within the UCYN-A1 clade dominating across varied environments. The biogeographic patterns and environmental correlations of and UCYN-A highlight the role of microdiversity in their ecological adaptation and reflect their different ecological strategies. These findings underscore the importance of characterizing the global patterns of fine-scale genetic diversity to better understand the functional roles and distribution of marine nitrogen-fixing photoautotrophs.IMPORTANCEThis study provides insights into the global diversity and distribution of nitrogen-fixing photoautotrophs, specifically and UCYN-A. We sequenced 954 oceanic samples of the nitrogenase gene and uncovered significant differences in microdiversity and environmental associations between these genera. showed high levels of sequence diversity and region-specific clades influenced by temperature and nutrient availability. In contrast, UCYN-A exhibited a more uniform distribution, thriving in iron-stressed regions. Quantifying these fine-scale genetic variations enhances our knowledge of their ecological roles and adaptations, emphasizing the need to characterize the genetic diversity of marine nitrogen-fixing prokaryotes.
title Global phylogeography and microdiversity of the marine diazotrophic photoautotrophs and UCYN-A.
topic Nitrogen Fixation
Phylogeography
Genetic Variation
Trichodesmium
Phylogeny
Seawater
Pacific Ocean
Indian Ocean
Nitrogenase
Atlantic Ocean
Biodiversity
url https://pubmed.ncbi.nlm.nih.gov/40643242/