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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40645544/ |
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Table of Contents:
- Roles of B7 homologue 3 protein (B7-H3) in T cell responses of large yellow croaker (Larimichthys crocea). Li, Qiuhua Chen, Yuhong Wang, Meiyan Li, Chenhao Ao, Jingqun Chen, Xinhua Animals Perciformes Fish Proteins B7 Antigens Phylogeny T-Lymphocytes Amino Acid Sequence Sequence Alignment Gene Expression Regulation Immunity, Innate Signal Transduction Gene Expression Profiling Base Sequence The B7 family provides critical co-stimulatory signals for T cell activation through receptor interactions, thereby modulating T cell proliferation and function. However, the roles and downstream signaling pathways of these molecules in teleosts remain largely unexplored. In the present study, we identified a B7-H3 homologue (LcB7-H3) in large yellow croaker (Larimichthys crocea), a commercially significant marine teleost species. The deduced LcB7-H3 protein contains conserved extracellular IgV-like and IgC-like domains, and phylogenetic analysis revealed its clustering with vertebrate B7-H3 homologues. It is constitutively expressed in all examined tissues and immune cells, with particularly high expression levels in mucosal tissues and primary head kidney monocytes/macrophages (PKMs). The recombinant LcB7-H3 protein (rLcB7-H3) significantly enhanced T cell proliferation and upregulated interleukin-2 expression, which is a hallmark of teleost T cell activation. Furthermore, rLcB7-H3 increased phosphorylation levels of MEK1/2 and ERK1/2 in the MAPK/ERK signaling pathway, as well as mTOR and 4E-BP1 in the mTOR signaling pathway. Collectively, these findings demonstrate that LcB7-H3 may promote T cell proliferation and activation via MAPK/ERK and mTOR pathways, suggesting evolutionarily conserved roles among vertebrate B7-H3 homologues.